Dendritic cells bearing HLA-G inhibit T-Cell activation in type 1 diabetes

• Published in: Iranian Journal of Allergy, Asthma and Immunology Asthma and Immunology, 2007-03, Vol.6 (1), p.1-7
• Main Authors: Abediankenari, Saeid, Eslami, Mohammad Bagher, Sarrafnejad, Abdolfattah, Mohseni, Mehrnosh, Larijani, Bagher
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Dendritic cell; Dendritic Cells - immunology; Diabetes Mellitus, Type 1 - immunology; Female; Histocompatibility Antigens Class I - physiology; HLA Antigens - physiology; HLA-G; HLA-G Antigens; Humans; IFN-β (Interferon β); Interferon-beta - pharmacology; Lymphocyte Activation; Male; Middle Aged; T-Lymphocytes - immunology
• ISSN: 1735-1502; 1365-2567; 1735-5249

HLA-G is normally expressed on human trophoblast cells. It is a non-classical MHC molecule class I b. The role of HLA-G in diabetic type 1 is not known. We investigated the role of IFN-beta in induction HLA-G expression on the monocyte derived dendritic cells (DC) in diabetes type 1. Treatment of dendritic cell with IFN-beta in vitro from diabetic patients (n=20) and normal subjects (n=20) resulted to the production and expression of HLA-G on these cells from both groups. However, comparison of DC from the diabetic patients with DC from the controls revealed lower levels of HLA-G molecules in DC from diabetic patients. Using mixed lymphocyte reaction (MLR), it was found that DC expressing HLA-G mediated the inhibition of autologous T cell activation. It is concluded that IFN-beta can increase HLA-G in DC from diabetic patients; subsequently it may prevent the immune regularly pathway in the diabetic pathogenesis.