Artificial Heme Enzymes for the Construction of Gold-Based Biomaterials

Many efforts are continuously devoted to the construction of hybrid biomaterials for specific applications, by immobilizing enzymes on different types of surfaces and/or nanomaterials. In addition, advances in computational, molecular and structural biology have led to a variety of strategies for de...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-09, Vol.19 (10), p.2896
Main Authors: Zambrano, Gerardo, Ruggiero, Emmanuel, Malafronte, Anna, Chino, Marco, Maglio, Ornella, Pavone, Vincenzo, Nastri, Flavia, Lombardi, Angela
Format: Article
Language:English
Subjects:
artificial enzymes
Biocompatible Materials - chemistry
Biocompatible Materials - metabolism
Catalysis
catalytic activity
Electrodes
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
functional biomaterials
Gold - chemistry
gold nanoparticles
Heme - chemistry
immobilization strategies
Metal Nanoparticles - chemistry
Oxidation-Reduction
peroxidases
Thioctic Acid - metabolism
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Summary:Many efforts are continuously devoted to the construction of hybrid biomaterials for specific applications, by immobilizing enzymes on different types of surfaces and/or nanomaterials. In addition, advances in computational, molecular and structural biology have led to a variety of strategies for designing and engineering artificial enzymes with defined catalytic properties. Here, we report the conjugation of an artificial heme enzyme (MIMO) with lipoic acid (LA) as a building block for the development of gold-based biomaterials. We show that the artificial MIMO@LA can be successfully conjugated to gold nanoparticles or immobilized onto gold electrode surfaces, displaying quasi-reversible redox properties and peroxidase activity. The results of this work open interesting perspectives toward the development of new totally-synthetic catalytic biomaterials for application in biotechnology and biomedicine, expanding the range of the biomolecular component aside from traditional native enzymes.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms19102896