Insight into the Phytoremediation Capability of Brassica juncea (v. Malopolska): Metal Accumulation and Antioxidant Enzyme Activity

• Published in: International journal of molecular sciences 2019-09, Vol.20 (18), p.4355
• Main Authors: Małecka, Arleta, Konkolewska, Agnieszka, Hanć, Anetta, Barałkiewicz, Danuta, Ciszewska, Liliana, Ratajczak, Ewelina, Staszak, Aleksandra Maria, Kmita, Hanna, Jarmuszkiewicz, Wiesława
• Format: Article
• Language: English
• Subjects: Antioxidants; Antioxidants - metabolism; antioxidative system; Biodegradation, Environmental; Biomass; Brassicaceae family; Cadmium; Carbohydrate metabolism; Carbohydrates; Chlorophyll; Chlorosis; Environmental conditions; Environmental impact; Enzymatic activity; Enzyme Activation; Enzyme activity; Enzymes; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; heavy metals; Leaves; Metals; Metals - metabolism; Metals, Heavy - metabolism; Micronutrients; Morphology; Mustard Plant - physiology; Nutrition; Organs; Oxidative Stress; Photosynthesis; Photosynthetic apparatus; Phytoremediation; Proteins; Reactive Oxygen Species - metabolism; Seedlings; Toxicity; Trace elements; Trace metals; Transpiration
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20184355

Metal hyperaccumulating plants should have extremely efficient defense mechanisms, enabling growth and development in a polluted environment. species are known to display hyperaccumulation capability. (Indiana mustard) v. Malopolska plants were exposed to trace elements, i.e., cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn), at a concentration of 50 μM and were then harvested after 96 h for analysis. We observed a high index of tolerance (IT), higher than 90%, for all plants treated with the four metals, and we showed that Cd, Cu, Pb, and Zn accumulation was higher in the above-ground parts than in the roots. We estimated the metal effects on the generation of reactive oxygen species (ROS) and the levels of protein oxidation, as well as on the activity and gene expression of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). The obtained results indicate that organo-specific ROS generation was higher in plants exposed to essential metal elements (i.e., Cu and Zn), compared with non-essential ones (i.e., Cd and Pb), in conjunction with SOD, CAT, and APX activity and expression at the level of encoding mRNAs and existing proteins. In addition to the potential usefulness of in the phytoremediation process, the data provide important information concerning plant response to the presence of trace metals.