Three-dimensional human placenta-like bud synthesized from induced pluripotent stem cells

Placental dysfunction is related to the pathogenesis of preeclampsia and fetal growth restriction, but there is no effective treatment for it. Recently, various functional three-dimensional organs have been generated from human induced-pluripotent cells (iPSCs), and the transplantation of these iPSC...

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Bibliographic Details
Published in:Scientific reports 2021-07, Vol.11 (1), p.14167-11, Article 14167
Main Authors: Sato, Mai, Inohaya, Asako, Yasuda, Eriko, Mogami, Haruta, Chigusa, Yoshitsugu, Kawasaki, Kaoru, Kawamura, Yosuke, Ueda, Yusuke, Takai, Hiroshi, Mandai, Masaki, Kondoh, Eiji
Format: Article
Language:English
Subjects:
631/136/2444
631/532
Achievement tests
Animal models
Animals
Antigens
Biomarkers - metabolism
Bone Morphogenetic Protein 4 - pharmacology
Cell Differentiation - drug effects
Cell Line
Cell Lineage - drug effects
Cytokeratin
Diabetes mellitus
Female
Fetuses
Humanities and Social Sciences
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - drug effects
Liver diseases
Mice
Mice, Inbred NOD
Mice, SCID
multidisciplinary
Placenta
Placenta - cytology
Placenta - drug effects
Placenta - transplantation
Pluripotency
Pre-eclampsia
Preeclampsia
Pregnancy
Proteins
Science
Science (multidisciplinary)
Stem cell transplantation
Stem cells
Trophoblasts
Trophoblasts - cytology
Trophoblasts - drug effects
Trophoblasts - metabolism
Uterus
Uterus - physiology
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Summary:Placental dysfunction is related to the pathogenesis of preeclampsia and fetal growth restriction, but there is no effective treatment for it. Recently, various functional three-dimensional organs have been generated from human induced-pluripotent cells (iPSCs), and the transplantation of these iPSCs-derived organs has alleviated liver failure or diabetes mellitus in mouse models. Here we successfully generated a three-dimensional placental organ bud from human iPSCs. The iPSCs differentiated into various lineages of trophoblasts such as cytotrophoblast-like, syncytiotrophoblast-like, and extravillous trophoblast-like cells, forming organized layers in the bud. Placental buds were transplanted to the murine uterus, where 22% of the buds were successfully engrafted. These iPSC-derived placental organ buds could serve as a new model for the study of placental function and pathology.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-93766-9