Cytotoxicity and Anti-Plasmodium berghei Activity of Emodin Loaded Nanoemulsion

Background: Malaria parasites cause a tremendous burden of disease in both the tropics and subtropics areas. Growing of drugs resistance in parasites is one of the most threats to malaria control. The aim of study was to investigate the anti-malarial activity of nano-emodin isolated from Rhamnus cat...

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Published in:Iranian journal of parasitology 2022-07, Vol.17 (3), p.339
Main Authors: Bayat, Fatemeh, Motevalli Haghi, Afsaneh, Nateghpour, Mehdi, Rahimi-Esboei, Bahman, Foroushani, Abbas Rahimi, Amani, Amir, Farivar, Leila, Talaee, Zahra Sayyad, Faryabi, Aref
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In-vivo test
Malaria
Nano emodin
Parasites
Plasmodium berghei
Rhamnus cathartica
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container_title Iranian journal of parasitology
container_volume 17
creator Bayat, Fatemeh
Motevalli Haghi, Afsaneh
Nateghpour, Mehdi
Rahimi-Esboei, Bahman
Foroushani, Abbas Rahimi
Amani, Amir
Farivar, Leila
Talaee, Zahra Sayyad
Faryabi, Aref
description Background: Malaria parasites cause a tremendous burden of disease in both the tropics and subtropics areas. Growing of drugs resistance in parasites is one of the most threats to malaria control. The aim of study was to investigate the anti-malarial activity of nano-emodin isolated from Rhamnus cathartica on Plasmodium berghei in mice to evaluate parasites inhibition rate using in-vivo test. Methods: The study was conducted in the School of Public Health, Tehran University of Medical Sciences, during 2020. Nano- emodin particles were prepared from Rhamnus cathartica, and confirmed by Zeta Potential Analyzer, DLS and electron microscopy techniques. Mice were infected with P. berghei and treated by emodin nanoparticles. Parasitemia was evaluated in each group in comparison with control group. Toxicity test was done using twice the highest concentration of emodin extract on a separate group of mice and ED50 was calculated. Results: Emodin extract was significantly effective in all concentrations on D4 (P
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Growing of drugs resistance in parasites is one of the most threats to malaria control. The aim of study was to investigate the anti-malarial activity of nano-emodin isolated from Rhamnus cathartica on Plasmodium berghei in mice to evaluate parasites inhibition rate using in-vivo test. Methods: The study was conducted in the School of Public Health, Tehran University of Medical Sciences, during 2020. Nano- emodin particles were prepared from Rhamnus cathartica, and confirmed by Zeta Potential Analyzer, DLS and electron microscopy techniques. Mice were infected with P. berghei and treated by emodin nanoparticles. Parasitemia was evaluated in each group in comparison with control group. Toxicity test was done using twice the highest concentration of emodin extract on a separate group of mice and ED50 was calculated. Results: Emodin extract was significantly effective in all concentrations on D4 (P&lt;0.05). The most effective on parasitemia was observed in 400 mg/kg of Liquid Nano-emodin and solid (non-Nano) emodin. ED50 for emodin extract was determined 220 mg/kg. Toxicity test showed no toxic effect on the subjects. Conclusion: The emodin extract is safe, lack of side effects. So, it can be used for more and longer period of time and in higher doses. Emodin extract, either in form of liquid and nanoparticle or in a solid form, has the same therapeutic effect on P. berghei in infected Balb/c mice.</description><identifier>ISSN: 1735-7020</identifier><identifier>EISSN: 2008-238X</identifier><identifier>DOI: 10.18502/ijpa.v17i3.10624</identifier><language>eng</language><publisher>Tehran: Tehran University of Medical Sciences</publisher><subject>In-vivo test ; Malaria ; Nano emodin ; Parasites ; Plasmodium berghei ; Rhamnus cathartica</subject><ispartof>Iranian journal of parasitology, 2022-07, Vol.17 (3), p.339</ispartof><rights>2022. 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subjects In-vivo test
Malaria
Nano emodin
Parasites
Plasmodium berghei
Rhamnus cathartica
title Cytotoxicity and Anti-Plasmodium berghei Activity of Emodin Loaded Nanoemulsion
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