Toxicity Effect of Silver Nanoparticles in Brine Shrimp Artemia

The present study revealed the toxic effect of silver nanoparticles (AgNPs) in Artemia nauplii and evaluated the mortality rate, hatching percentage, and genotoxic effect in Artemia nauplii/cysts. The AgNPs were commercially purchased and characterized using field emission scanning electron microsco...

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Bibliographic Details
Published in:TheScientificWorld 2014-01, Vol.2014 (2014), p.1-10
Main Authors: Chandhirasekar, Devakumar, Prabhu, Durai, Jennifer, Samou Michael, Arulvasu, Chinnasamy
Format: Article
Language:English
Subjects:
Animals
Artemia
Artemia - drug effects
Bioassays
Chemical analysis
Colleges & universities
Cysts
Deoxyribonucleic acid
DNA
Hatching
Health aspects
Laboratories
Medical wastes
Metal Nanoparticles - chemistry
Metal Nanoparticles - toxicity
Molecular weight
Mortality
Nanoparticles
Nanotechnology
Salt flats
Seawater
Silver
Silver - chemistry
Silver - toxicity
Sodium
Toxicity
Water analysis
X-ray spectroscopy
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Summary:The present study revealed the toxic effect of silver nanoparticles (AgNPs) in Artemia nauplii and evaluated the mortality rate, hatching percentage, and genotoxic effect in Artemia nauplii/cysts. The AgNPs were commercially purchased and characterized using field emission scanning electron microscope with energy dispersive X-ray spectroscopy. Nanoparticles were spherical in nature and with size range of 30–40 nm. Artemia cysts were collected from salt pan, processed, and hatched in sea water. Artemia nauplii (II instar) were treated using silver nanoparticles of various nanomolar concentrations and LC50 value (10 nM) and mortality rate (24 and 48 hours) was evaluated. Hatching percentage of decapsulated cysts treated with AgNPs was examined. Aggregation of AgNPs in the gut region of nauplii was studied using phase contrast microscope and apoptotic cells in nauplii stained with acridine orange were observed using fluorescence microscope. DNA damage of single cell of nauplii was determined by comet assay. This study showed that as the concentration of AgNPs increased, the mortality rate, aggregation in gut region, apoptotic cells, and DNA damage increased in nauplii, whereas the percentage of hatching in Artemia cysts decreased. Thus this study revealed that the nanomolar concentrations of AgNPs have toxic effect on both Artemia nauplii and cysts.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/256919