Development of 18 microsatellite markers for Atractylodes japonica

Premise Atractylodes japonica (Asteraceae) is endemic to East Asia, where its rhizomes are used in traditional medicine. To investigate the genetic diversity of this species, we developed polymorphic microsatellite markers. Methods and Results We obtained a total of 175,825 simple sequence repeat (S...

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Bibliographic Details
Published in:Applications in plant sciences 2020-05, Vol.8 (5), p.e11350-n/a
Main Authors: Jeong, Jin‐Tae, Chung, Hee, Ha, Bo‐Keun, Gil, Jinsu, Lee, Jeong‐Hoon, Lee, Yun‐Ji, Kim, Mi Ran, Oh, MyeongWon, Park, Chun Geon, Chang, Jae Ki, Hong, Chang Pyo, Park, Sin‐Gi, Lee, Yi
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Asteraceae
Atractylodes japonica
Deoxyribonucleic acid
DNA
Genetic diversity
Genetic markers
Genetic testing
Genomes
Herbal medicine
Heterozygosity
microsatellite markers
Microsatellites
Plant sciences
Population genetics
Population studies
Primer Note
Primer Notes
Rhizomes
simple sequence repeat (SSR)
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Summary:Premise Atractylodes japonica (Asteraceae) is endemic to East Asia, where its rhizomes are used in traditional medicine. To investigate the genetic diversity of this species, we developed polymorphic microsatellite markers. Methods and Results We obtained a total of 175,825 simple sequence repeat (SSR) loci using the Illumina HiSeq 2500 system. Eighteen polymorphic SSR primer pairs were selected to determine heterozygosity levels and allele numbers in 80 individuals from four A. japonica populations. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.133 to 0.892, respectively. Cross‐amplification in the related species A. macrocephala and A. lancea was successful in 15 and 14 of the 18 markers, respectively. Conclusions These microsatellite markers will be useful for future studies involving A. japonica population genetics and breeding.
ISSN:2168-0450
2168-0450
DOI:10.1002/aps3.11350