Leaves of Cedrela sinensis Attenuate Chronic Unpredictable Mild Stress-Induced Depression-like Behavior via Regulation of Hormonal and Inflammatory Imbalance

• Published in: Antioxidants 2022-12, Vol.11 (12), p.2448
• Main Authors: Jeong, Hye Rin, Kim, Jong Min, Lee, Uk, Kang, Jin Yong, Park, Seon Kyeong, Lee, Hyo Lim, Moon, Jong Hyun, Kim, Min Ji, Go, Min Ji, Heo, Ho Jin
• Format: Article
• Language: English
• Subjects: Acetic acid; Acids; Adaptation; Adrenocorticotropic hormone; Apoptosis; Bax protein; Behavior; Brain-derived neurotrophic factor; Caspase-1; Cedrela sinensis; chronic unpredictable mild stress; Corticosterone; Corticotropin-releasing hormone; Cytokines; depression; Drinking water; Energy metabolism; Ethyl acetate; Experiments; hormone changes; Hydrogen peroxide; IL-1β; Inflammation; Kaempferol; Membrane potential; Mental depression; Mitochondria; mitochondrial function; Neuroblastoma; Neuroprotection; Open-field behavior; Oxidative stress; Reactive oxygen species; Rutin; Steroid 11β-hydroxylase; Sucrose; Tau protein; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox11122448

This study aimed to evaluate the protective effects of ethyl acetate fraction from (EFCS) against chronic unpredictable mild stress (CUMS)-induced behavioral dysfunction and stress response in C57BL/6 mice. The physiological compounds of EFCS were identified as rutin, isoquercitrin, ethyl gallate, quercitrin, kaempferol-3-O-rhamnoside, and ethyl digallate, using UPLC-Q-TOF/MS . To evaluate the neuroprotective effect of EFCS, H O and corticosterone-induced neuronal cell viability was conducted in human neuroblastoma MC-IXC cells. It was found that EFCS alleviated depression-like behavior by conducting the sucrose preference test (SPT), forced swimming test (FST), open field test (OFT), and tail suspension test (TST). EFCS inhibited mitochondrial dysfunction related to neuronal energy metabolism by regulating reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), and ATP contents in brain tissue. In addition, the administration of EFCS regulated the stress hormones in serum. EFCS regulated stress-related indicators such as CRF, ACTH, CYP11B1, and BDNF. Moreover, EFCS downregulated the inflammatory responses and apoptosis proteins such as caspase-1, TNF-α, IL-1β, p-JNK, BAX, and p-tau in brain tissues. These results suggest that EFCS might be a potential natural plant material that alleviates CUMS-induced behavior disorder by regulating inflammation in brain tissue against CUMS-induced depression.