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Bacillus subtilis natto Derivatives Inhibit Enterococcal Biofilm Formation via Restructuring of the Cell Envelope
is considered a leading cause of hospital-acquired infections. Treatment of these infections has become a major challenge for clinicians because some strains are resistant to multiple clinically used antibiotics. Moreover, the presence of biofilms can make infections with more difficult to eradicate...
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Published in: | Frontiers in microbiology 2021-12, Vol.12, p.785351-785351 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | is considered a leading cause of hospital-acquired infections. Treatment of these infections has become a major challenge for clinicians because some
strains are resistant to multiple clinically used antibiotics. Moreover, the presence of
biofilms can make infections with
more difficult to eradicate with current antibiotic therapies. Thus, our aim in this study was to investigate the effects of probiotic derivatives against
biofilm formation.
natto is a probiotic strain isolated from Japanese fermented soybean foods, and its culture fluid potently inhibited adherence to Caco-2 cell monolayers, aggregation, and biofilm production without inhibiting the growth of
. An apparent decrease in the thickness of
biofilms was observed through confocal laser scanning microscopy. In addition, exopolysaccharide synthesis in
biofilms was reduced by
natto culture fluid treatment. Carbohydrate composition analysis also showed that carbohydrates in the
cell envelope were restructured. Furthermore, transcriptome sequencing revealed that the culture fluid of
natto downregulated the transcription of genes involved in the WalK/WalR two-component system, peptidoglycan biosynthesis and membrane glycolipid biosynthesis, which are all crucial for
cell envelope synthesis and biofilm formation. Collectively, our work shows that some derivatives present in the culture fluid of
natto may be useful for controlling
biofilms. |
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ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2021.785351 |