The French press: a repeatable and high-throughput approach to exercising zebrafish ( Danio rerio )

Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafi...

Full description

Saved in:
Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2018-01, Vol.6, p.e4292-e4292, Article e4292
Main Authors: Usui, Takuji, Noble, Daniel W A, O'Dea, Rose E, Fangmeier, Melissa L, Lagisz, Malgorzata, Hesselson, Daniel, Nakagawa, Shinichi
Format: Article
Language:English
Subjects:
Analysis
Animal Behavior
Aquaculture, Fisheries and Fish Science
Biology
Burst swimming
Circular
Coffee
Danio rerio
Ecology
Endurance test
Exercise
Experiments
Fish
Fitness training programs
Gene expression
Genetic aspects
Medical research
Metabolism
Obesity
Physiological aspects
Physiology
Studies
Sustained swimming
Swimming
Technique
Vortices
Weight control
Zebrafish
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Zebrafish are increasingly used as a vertebrate model organism for various traits including swimming performance, obesity and metabolism, necessitating high-throughput protocols to generate standardized phenotypic information. Here, we propose a novel and cost-effective method for exercising zebrafish, using a coffee plunger and magnetic stirrer. To demonstrate the use of this method, we conducted a pilot experiment to show that this simple system provides repeatable estimates of maximal swim performance (intra-class correlation [ICC] = 0.34-0.41) and observe that exercise training of zebrafish on this system significantly increases their maximum swimming speed. We propose this high-throughput and reproducible system as an alternative to traditional linear chamber systems for exercising zebrafish and similarly sized fishes.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.4292