Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus Inhibition of the growth of Escherichia coli isolated from 'Minas Frescal' cheese by Lactobacillus acidophilus

Escherichia coli faz parte da microbiota anaeróbica facultativa normal, sendo também considerada um dos maiores patógenos entéricos predominantes no cólon dos animais e homem. Neste trabalho, realizaram-se ensaios "in vitro" para avaliar o grau de atividade antagonista de cinco cepas de La...

Full description

Saved in:
Bibliographic Details
Published in:Ciência rural 2007-04, Vol.37 (2), p.583-585
Main Authors: Tammy Priscilla Chioda, Rubén Pablo Schocken-Iturrino, Gisela Rojas Garcia, Caroline Peters Pigatto, César Augusto Martins Ribeiro, Adriana Valim Ferreira Ragazzani
Format: Article
Language:English
Subjects:
antagonistic action
atividade antagonista
bactéria ácido-lática
lactic acid bacteria
pathogens
patógenos
probiotic
probiótico
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 585
container_issue 2
container_start_page 583
container_title Ciência rural
container_volume 37
creator Tammy Priscilla Chioda
Rubén Pablo Schocken-Iturrino
Gisela Rojas Garcia
Caroline Peters Pigatto
César Augusto Martins Ribeiro
Adriana Valim Ferreira Ragazzani
description Escherichia coli faz parte da microbiota anaeróbica facultativa normal, sendo também considerada um dos maiores patógenos entéricos predominantes no cólon dos animais e homem. Neste trabalho, realizaram-se ensaios "in vitro" para avaliar o grau de atividade antagonista de cinco cepas de Lactobacillus acidophilus, com capacidade probiótica sobre Escherichia coli BIA 26 (STEC) isolada de queijo "Minas Frescal". Para tanto, foi utilizado o teste de inibição através do método de dupla camada em triplicata para avaliar zonas de inibição de crescimento. Todas as cepas de Lactobacillus mostraram-se capazes de inibir a E. coli, com zonas de inibição variando de 12 a 15mm de diâmetro, sendo que a maioria apresentou 14mm de diâmetro, consideradas como fortes halos de inibição.Escherichia coli is part of the normal facultative anaerobic microflora and one of the major enteric pathogens in the colon of animals and humans. In vitro trials were conducted to evaluate the level of antagonistic activity of five strains of Lactobacillus acidophilus with probiotic effect on Escherichia coli BIA 26 (STEC), which was isolated from 'Minas frescal' cheese. Inhibition assays were carried out using the double layer method in triplicates to evaluate growth inhibition zones. All L. acidophilus strains were capable to inhibit E. coli, with inhibition zones ranging from 12 to 15mm, but mostly around 14mm in diameter, and considered strong inhibition halos.
doi_str_mv 10.1590/S0103-84782007000200048
format article
fullrecord <record><control><sourceid>doaj</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad21</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad21</doaj_id><sourcerecordid>oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad21</sourcerecordid><originalsourceid>FETCH-doaj_primary_oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad213</originalsourceid><addsrcrecordid>eNqtjc9Kw0AQxhdRsP55BodeeoruNpsmOUuLBT2I3sPuZtJMSTNlN0X6PB58kL6GD2MiIgh68_TNN_PN9xPiSslrleTy5kkqGUeZTrOplKmUshepsyMxUrM0i3SSz47F6Dt0Ks5CWPepNNZ6JN6XLVk6vB1eGUoG5zE42mDb9RZhHlyNnlxNBhw3BBS4MaUZbo87pDXD-IFaE2AxPJpmDFv2cG9cx9Y4appdgF5L3tY0zMu27nEdcQtcQVcjrDy_dPXgfod1WELleQOTH6AJ9GEMCHb_N-5CnFSmCXj5pediuZg_395FJZt1sfW0MX5fsKHic8F-VRjfkWuwsDY2icZkijLTyqYWMa5SlyvtclNOVfyfXR8AnZX6</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus Inhibition of the growth of Escherichia coli isolated from 'Minas Frescal' cheese by Lactobacillus acidophilus</title><source>SciELO Brazil</source><creator>Tammy Priscilla Chioda ; Rubén Pablo Schocken-Iturrino ; Gisela Rojas Garcia ; Caroline Peters Pigatto ; César Augusto Martins Ribeiro ; Adriana Valim Ferreira Ragazzani</creator><creatorcontrib>Tammy Priscilla Chioda ; Rubén Pablo Schocken-Iturrino ; Gisela Rojas Garcia ; Caroline Peters Pigatto ; César Augusto Martins Ribeiro ; Adriana Valim Ferreira Ragazzani</creatorcontrib><description>Escherichia coli faz parte da microbiota anaeróbica facultativa normal, sendo também considerada um dos maiores patógenos entéricos predominantes no cólon dos animais e homem. Neste trabalho, realizaram-se ensaios "in vitro" para avaliar o grau de atividade antagonista de cinco cepas de Lactobacillus acidophilus, com capacidade probiótica sobre Escherichia coli BIA 26 (STEC) isolada de queijo "Minas Frescal". Para tanto, foi utilizado o teste de inibição através do método de dupla camada em triplicata para avaliar zonas de inibição de crescimento. Todas as cepas de Lactobacillus mostraram-se capazes de inibir a E. coli, com zonas de inibição variando de 12 a 15mm de diâmetro, sendo que a maioria apresentou 14mm de diâmetro, consideradas como fortes halos de inibição.Escherichia coli is part of the normal facultative anaerobic microflora and one of the major enteric pathogens in the colon of animals and humans. In vitro trials were conducted to evaluate the level of antagonistic activity of five strains of Lactobacillus acidophilus with probiotic effect on Escherichia coli BIA 26 (STEC), which was isolated from 'Minas frescal' cheese. Inhibition assays were carried out using the double layer method in triplicates to evaluate growth inhibition zones. All L. acidophilus strains were capable to inhibit E. coli, with inhibition zones ranging from 12 to 15mm, but mostly around 14mm in diameter, and considered strong inhibition halos.</description><identifier>ISSN: 0103-8478</identifier><identifier>EISSN: 1678-4596</identifier><identifier>DOI: 10.1590/S0103-84782007000200048</identifier><language>eng</language><publisher>Universidade Federal de Santa Maria</publisher><subject>antagonistic action ; atividade antagonista ; bactéria ácido-lática ; lactic acid bacteria ; pathogens ; patógenos ; probiotic ; probiótico</subject><ispartof>Ciência rural, 2007-04, Vol.37 (2), p.583-585</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Tammy Priscilla Chioda</creatorcontrib><creatorcontrib>Rubén Pablo Schocken-Iturrino</creatorcontrib><creatorcontrib>Gisela Rojas Garcia</creatorcontrib><creatorcontrib>Caroline Peters Pigatto</creatorcontrib><creatorcontrib>César Augusto Martins Ribeiro</creatorcontrib><creatorcontrib>Adriana Valim Ferreira Ragazzani</creatorcontrib><title>Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus Inhibition of the growth of Escherichia coli isolated from 'Minas Frescal' cheese by Lactobacillus acidophilus</title><title>Ciência rural</title><description>Escherichia coli faz parte da microbiota anaeróbica facultativa normal, sendo também considerada um dos maiores patógenos entéricos predominantes no cólon dos animais e homem. Neste trabalho, realizaram-se ensaios "in vitro" para avaliar o grau de atividade antagonista de cinco cepas de Lactobacillus acidophilus, com capacidade probiótica sobre Escherichia coli BIA 26 (STEC) isolada de queijo "Minas Frescal". Para tanto, foi utilizado o teste de inibição através do método de dupla camada em triplicata para avaliar zonas de inibição de crescimento. Todas as cepas de Lactobacillus mostraram-se capazes de inibir a E. coli, com zonas de inibição variando de 12 a 15mm de diâmetro, sendo que a maioria apresentou 14mm de diâmetro, consideradas como fortes halos de inibição.Escherichia coli is part of the normal facultative anaerobic microflora and one of the major enteric pathogens in the colon of animals and humans. In vitro trials were conducted to evaluate the level of antagonistic activity of five strains of Lactobacillus acidophilus with probiotic effect on Escherichia coli BIA 26 (STEC), which was isolated from 'Minas frescal' cheese. Inhibition assays were carried out using the double layer method in triplicates to evaluate growth inhibition zones. All L. acidophilus strains were capable to inhibit E. coli, with inhibition zones ranging from 12 to 15mm, but mostly around 14mm in diameter, and considered strong inhibition halos.</description><subject>antagonistic action</subject><subject>atividade antagonista</subject><subject>bactéria ácido-lática</subject><subject>lactic acid bacteria</subject><subject>pathogens</subject><subject>patógenos</subject><subject>probiotic</subject><subject>probiótico</subject><issn>0103-8478</issn><issn>1678-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqtjc9Kw0AQxhdRsP55BodeeoruNpsmOUuLBT2I3sPuZtJMSTNlN0X6PB58kL6GD2MiIgh68_TNN_PN9xPiSslrleTy5kkqGUeZTrOplKmUshepsyMxUrM0i3SSz47F6Dt0Ks5CWPepNNZ6JN6XLVk6vB1eGUoG5zE42mDb9RZhHlyNnlxNBhw3BBS4MaUZbo87pDXD-IFaE2AxPJpmDFv2cG9cx9Y4appdgF5L3tY0zMu27nEdcQtcQVcjrDy_dPXgfod1WELleQOTH6AJ9GEMCHb_N-5CnFSmCXj5pediuZg_395FJZt1sfW0MX5fsKHic8F-VRjfkWuwsDY2icZkijLTyqYWMa5SlyvtclNOVfyfXR8AnZX6</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Tammy Priscilla Chioda</creator><creator>Rubén Pablo Schocken-Iturrino</creator><creator>Gisela Rojas Garcia</creator><creator>Caroline Peters Pigatto</creator><creator>César Augusto Martins Ribeiro</creator><creator>Adriana Valim Ferreira Ragazzani</creator><general>Universidade Federal de Santa Maria</general><scope>DOA</scope></search><sort><creationdate>20070401</creationdate><title>Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus Inhibition of the growth of Escherichia coli isolated from 'Minas Frescal' cheese by Lactobacillus acidophilus</title><author>Tammy Priscilla Chioda ; Rubén Pablo Schocken-Iturrino ; Gisela Rojas Garcia ; Caroline Peters Pigatto ; César Augusto Martins Ribeiro ; Adriana Valim Ferreira Ragazzani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-doaj_primary_oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>antagonistic action</topic><topic>atividade antagonista</topic><topic>bactéria ácido-lática</topic><topic>lactic acid bacteria</topic><topic>pathogens</topic><topic>patógenos</topic><topic>probiotic</topic><topic>probiótico</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tammy Priscilla Chioda</creatorcontrib><creatorcontrib>Rubén Pablo Schocken-Iturrino</creatorcontrib><creatorcontrib>Gisela Rojas Garcia</creatorcontrib><creatorcontrib>Caroline Peters Pigatto</creatorcontrib><creatorcontrib>César Augusto Martins Ribeiro</creatorcontrib><creatorcontrib>Adriana Valim Ferreira Ragazzani</creatorcontrib><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Ciência rural</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tammy Priscilla Chioda</au><au>Rubén Pablo Schocken-Iturrino</au><au>Gisela Rojas Garcia</au><au>Caroline Peters Pigatto</au><au>César Augusto Martins Ribeiro</au><au>Adriana Valim Ferreira Ragazzani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus Inhibition of the growth of Escherichia coli isolated from 'Minas Frescal' cheese by Lactobacillus acidophilus</atitle><jtitle>Ciência rural</jtitle><date>2007-04-01</date><risdate>2007</risdate><volume>37</volume><issue>2</issue><spage>583</spage><epage>585</epage><pages>583-585</pages><issn>0103-8478</issn><eissn>1678-4596</eissn><abstract>Escherichia coli faz parte da microbiota anaeróbica facultativa normal, sendo também considerada um dos maiores patógenos entéricos predominantes no cólon dos animais e homem. Neste trabalho, realizaram-se ensaios "in vitro" para avaliar o grau de atividade antagonista de cinco cepas de Lactobacillus acidophilus, com capacidade probiótica sobre Escherichia coli BIA 26 (STEC) isolada de queijo "Minas Frescal". Para tanto, foi utilizado o teste de inibição através do método de dupla camada em triplicata para avaliar zonas de inibição de crescimento. Todas as cepas de Lactobacillus mostraram-se capazes de inibir a E. coli, com zonas de inibição variando de 12 a 15mm de diâmetro, sendo que a maioria apresentou 14mm de diâmetro, consideradas como fortes halos de inibição.Escherichia coli is part of the normal facultative anaerobic microflora and one of the major enteric pathogens in the colon of animals and humans. In vitro trials were conducted to evaluate the level of antagonistic activity of five strains of Lactobacillus acidophilus with probiotic effect on Escherichia coli BIA 26 (STEC), which was isolated from 'Minas frescal' cheese. Inhibition assays were carried out using the double layer method in triplicates to evaluate growth inhibition zones. All L. acidophilus strains were capable to inhibit E. coli, with inhibition zones ranging from 12 to 15mm, but mostly around 14mm in diameter, and considered strong inhibition halos.</abstract><pub>Universidade Federal de Santa Maria</pub><doi>10.1590/S0103-84782007000200048</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0103-8478
ispartof Ciência rural, 2007-04, Vol.37 (2), p.583-585
issn 0103-8478
1678-4596
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad21
source SciELO Brazil
subjects antagonistic action
atividade antagonista
bactéria ácido-lática
lactic acid bacteria
pathogens
patógenos
probiotic
probiótico
title Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus Inhibition of the growth of Escherichia coli isolated from 'Minas Frescal' cheese by Lactobacillus acidophilus
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T11%3A58%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-doaj&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inibi%C3%A7%C3%A3o%20do%20crescimento%20de%20Escherichia%20coli%20isolada%20de%20Queijo%20%22Minas%20Frescal%22%20por%20Lactobacillus%20acidophilus%20Inhibition%20of%20the%20growth%20of%20Escherichia%20coli%20isolated%20from%20'Minas%20Frescal'%20cheese%20by%20Lactobacillus%20acidophilus&rft.jtitle=Ci%C3%AAncia%20rural&rft.au=Tammy%20Priscilla%20Chioda&rft.date=2007-04-01&rft.volume=37&rft.issue=2&rft.spage=583&rft.epage=585&rft.pages=583-585&rft.issn=0103-8478&rft.eissn=1678-4596&rft_id=info:doi/10.1590/S0103-84782007000200048&rft_dat=%3Cdoaj%3Eoai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad21%3C/doaj%3E%3Cgrp_id%3Ecdi_FETCH-doaj_primary_oai_doaj_org_article_bb3a54e52e0841b7bee3f7c914c9ad213%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true