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Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae
The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like...
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| Published in: | Frontiers in veterinary science 2023-06, Vol.10, p.1197701-1197701 |
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| creator | Toquet, Marion Bataller, Esther Gomis, Jesús Sánchez, Antonio Toledo-Perona, Raquel De la Fe, Christian Corrales, Juan Carlos Gómez-Martín, Ángel |
| description | The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like
but never against
(Ma). This
study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of
spp.
A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium
, for an
experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 10
CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 10
to 8.4 × 10
CFU/mL.
The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (
|
| doi_str_mv | 10.3389/fvets.2023.1197701 |
| format | article |
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but never against
(Ma). This
study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of
spp.
A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium
, for an
experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 10
CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 10
to 8.4 × 10
CFU/mL.
The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (
< 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (
< 0.001), and 120B from 6.825 to 6.466 log CFU/mL (
< 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (
< 0.001).
This is the first
report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible
studies.</description><identifier>ISSN: 2297-1769</identifier><identifier>EISSN: 2297-1769</identifier><identifier>DOI: 10.3389/fvets.2023.1197701</identifier><identifier>PMID: 37426075</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>antimicrobial activity ; contagious agalactia ; Enterococcus ; Lactobacillus ; mastitis ; Mycoplasma agalactiae ; Veterinary Science</subject><ispartof>Frontiers in veterinary science, 2023-06, Vol.10, p.1197701-1197701</ispartof><rights>Copyright © 2023 Toquet, Bataller, Gomis, Sánchez, Toledo-Perona, De la Fe, Corrales and Gómez-Martín.</rights><rights>Copyright © 2023 Toquet, Bataller, Gomis, Sánchez, Toledo-Perona, De la Fe, Corrales and Gómez-Martín. 2023 Toquet, Bataller, Gomis, Sánchez, Toledo-Perona, De la Fe, Corrales and Gómez-Martín</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-7f9e42489a4addb7c95591e2e430cdef805e2ec85a9689d0cfd490b605e8118e3</citedby><cites>FETCH-LOGICAL-c469t-7f9e42489a4addb7c95591e2e430cdef805e2ec85a9689d0cfd490b605e8118e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324661/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324661/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37426075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Toquet, Marion</creatorcontrib><creatorcontrib>Bataller, Esther</creatorcontrib><creatorcontrib>Gomis, Jesús</creatorcontrib><creatorcontrib>Sánchez, Antonio</creatorcontrib><creatorcontrib>Toledo-Perona, Raquel</creatorcontrib><creatorcontrib>De la Fe, Christian</creatorcontrib><creatorcontrib>Corrales, Juan Carlos</creatorcontrib><creatorcontrib>Gómez-Martín, Ángel</creatorcontrib><title>Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae</title><title>Frontiers in veterinary science</title><addtitle>Front Vet Sci</addtitle><description>The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like
but never against
(Ma). This
study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of
spp.
A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium
, for an
experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 10
CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 10
to 8.4 × 10
CFU/mL.
The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (
< 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (
< 0.001), and 120B from 6.825 to 6.466 log CFU/mL (
< 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (
< 0.001).
This is the first
report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible
studies.</description><subject>antimicrobial activity</subject><subject>contagious agalactia</subject><subject>Enterococcus</subject><subject>Lactobacillus</subject><subject>mastitis</subject><subject>Mycoplasma agalactiae</subject><subject>Veterinary Science</subject><issn>2297-1769</issn><issn>2297-1769</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpVUstOHDEQHKFEgAg_wCHyMZfd-DkenyKEQoJExAXOVo8fGxPPeGPPLuI38sXx7C4ITq4ud1W3rWqaC4KXjHXqq9-6qSwppmxJiJISk6PmlFIlF0S26sMbfNKcl_KIMSaCS9bh4-aESU5bLMVp8-9ynEIPZnI5QETrNLlKVJQ8MmkYXDZzBaNFTyFaFGtrMAhMsOhFhsqUIYwFhZIiTM4in9OA0jaMbqc0sM4zzvCEhhD_IFjN_RP69WzSOkIZYKZ23uA-NR89xOLOD-dZ83D9_f7q5-L27sfN1eXtwvBWTQvpleOUdwo4WNtLo4RQxFHHGTbW-Q6LWphOgGo7ZbHxlivct5XuCOkcO2tu9r42waOuGw6Qn3WCoHdEyisNuT42Ot33nCqgQlqMueitMoTz3lPBjTHKs-r1be-13vSDs6Z-Yob4zvT9zRh-61XaaoIZ5W1LqsOXg0NOfzeuTHoIxbgYYXRpUzTtmKKCMj4Po_tWk1Mp2fnXOQTrORx6Fw49h0MfwlFFn99u-Cp5iQL7DxU2uq8</recordid><startdate>20230622</startdate><enddate>20230622</enddate><creator>Toquet, Marion</creator><creator>Bataller, Esther</creator><creator>Gomis, Jesús</creator><creator>Sánchez, Antonio</creator><creator>Toledo-Perona, Raquel</creator><creator>De la Fe, Christian</creator><creator>Corrales, Juan Carlos</creator><creator>Gómez-Martín, Ángel</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20230622</creationdate><title>Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae</title><author>Toquet, Marion ; Bataller, Esther ; Gomis, Jesús ; Sánchez, Antonio ; Toledo-Perona, Raquel ; De la Fe, Christian ; Corrales, Juan Carlos ; Gómez-Martín, Ángel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-7f9e42489a4addb7c95591e2e430cdef805e2ec85a9689d0cfd490b605e8118e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>antimicrobial activity</topic><topic>contagious agalactia</topic><topic>Enterococcus</topic><topic>Lactobacillus</topic><topic>mastitis</topic><topic>Mycoplasma agalactiae</topic><topic>Veterinary Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Toquet, Marion</creatorcontrib><creatorcontrib>Bataller, Esther</creatorcontrib><creatorcontrib>Gomis, Jesús</creatorcontrib><creatorcontrib>Sánchez, Antonio</creatorcontrib><creatorcontrib>Toledo-Perona, Raquel</creatorcontrib><creatorcontrib>De la Fe, Christian</creatorcontrib><creatorcontrib>Corrales, Juan Carlos</creatorcontrib><creatorcontrib>Gómez-Martín, Ángel</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Frontiers in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Toquet, Marion</au><au>Bataller, Esther</au><au>Gomis, Jesús</au><au>Sánchez, Antonio</au><au>Toledo-Perona, Raquel</au><au>De la Fe, Christian</au><au>Corrales, Juan Carlos</au><au>Gómez-Martín, Ángel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae</atitle><jtitle>Frontiers in veterinary science</jtitle><addtitle>Front Vet Sci</addtitle><date>2023-06-22</date><risdate>2023</risdate><volume>10</volume><spage>1197701</spage><epage>1197701</epage><pages>1197701-1197701</pages><issn>2297-1769</issn><eissn>2297-1769</eissn><abstract>The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like
but never against
(Ma). This
study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of
spp.
A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium
, for an
experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 10
CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 10
to 8.4 × 10
CFU/mL.
The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (
< 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (
< 0.001), and 120B from 6.825 to 6.466 log CFU/mL (
< 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (
< 0.001).
This is the first
report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible
studies.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>37426075</pmid><doi>10.3389/fvets.2023.1197701</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Frontiers in veterinary science, 2023-06, Vol.10, p.1197701-1197701 |
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| source | PMC (PubMed Central) |
| subjects | antimicrobial activity contagious agalactia Enterococcus Lactobacillus mastitis Mycoplasma agalactiae Veterinary Science |
| title | Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae |
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