Effects of Lactobacillus rhamnosus DSM7133 on Intestinal Porcine Epithelial Cells

Antimicrobial resistance is one of the biggest health challenges nowadays. Probiotics are promising candidates as feed additives contributing to the health of the gastrointestinal tract. The beneficial effect of probiotics is species/strain specific; the potential benefits need to be individually as...

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Published in:Animals (Basel) 2023-09, Vol.13 (19), p.3007
Main Authors: Palkovicsné Pézsa, Nikolett, Kovács, Dóra, Somogyi, Fanni, Karancsi, Zita, Móritz, Alma Virág, Jerzsele, Ákos, Rácz, Bence, Farkas, Orsolya
Format: Article
Language:English
Subjects:
Antibiotics
Antimicrobial agents
Bacteria
Bacterial infections
Cell culture
E coli
Escherichia coli
Experiments
Food chains
Hogs
Immune system
Infections
IPEC-J2
Kinases
Lactobacillus rhamnosus
Microbiota
paracellular permeability
Pathogens
Permeability
Physiology
Probiotics
Proteins
ROS
Salmonella
Salmonella enterica serovar Typhimurium
Signal transduction
Veterinary medicine
Weaning
Zoonoses
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Summary:Antimicrobial resistance is one of the biggest health challenges nowadays. Probiotics are promising candidates as feed additives contributing to the health of the gastrointestinal tract. The beneficial effect of probiotics is species/strain specific; the potential benefits need to be individually assessed for each probiotic strain or species. We established a co-culture model, in which gastrointestinal infection was modeled using Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. enterica serovar Typhimurium). Using intestinal porcine epithelial cells (IPEC-J2), the effects of pre-, co-, and post-treatment with Lactobacillus (L.) rhamnosus on the barrier function, intracellular (IC) reactive oxygen species (ROS) production, proinflammatory cytokine (IL-6 and IL-8) response, and adhesion inhibition were tested. E. coli- and S. Typhimurium-induced barrier impairment and increased ROS production could be counteracted using L. rhamnosus (p < 0.01). S. Typhimurium-induced IL-6 production was reduced via pre-treatment (p < 0.05) and post-treatment (p < 0.01); increased IL-8 secretion was decreased via pre-, co-, and post-treatment (p < 0.01) with L. rhamnosus. L. rhamnosus demonstrated significant inhibition of adhesion for both S. Typhimurium (p < 0.001) and E. coli (p < 0.001 in both pre-treatment and post-treatment; p < 0.05 in co-treatment). This study makes a substantial contribution to the understanding of the specific benefits of L. rhamnosus. Our findings can serve as a basis for further in vivo studies carried out in pigs and humans.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani13193007