Application of FTIR Spectroscopy to Detect Changes in Skeletal Muscle Composition Due to Obesity with Insulin Resistance and STZ-Induced Diabetes

Age, obesity, and diabetes mellitus are pathophysiologically interconnected factors that significantly contribute to the global burden of non-communicable diseases. These metabolic conditions are associated with impaired insulin function, which disrupts the metabolism of carbohydrates, lipids, and p...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-10, Vol.23 (20), p.12498
Main Authors: Zupančič, Barbara, Umek, Nejc, Ugwoke, Chiedozie Kenneth, Cvetko, Erika, Horvat, Simon, Grdadolnik, Jože
Format: Article
Language:English
Subjects:
Carbohydrates
Change detection
Composition
diabetes
Diabetes mellitus
Fourier transform infrared (FTIR) spectroscopy
histochemical analysis
Infrared spectroscopy
Insulin
Insulin resistance
Lipid metabolism
Lipids
Macromolecules
Muscles
Obesity
Phenotypes
Skeletal muscle
skeletal muscle macromolecular composition
Spectrum analysis
Streptozocin
Structure-function relationships
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Summary:Age, obesity, and diabetes mellitus are pathophysiologically interconnected factors that significantly contribute to the global burden of non-communicable diseases. These metabolic conditions are associated with impaired insulin function, which disrupts the metabolism of carbohydrates, lipids, and proteins and can lead to structural and functional changes in skeletal muscle. Therefore, the alterations in the macromolecular composition of skeletal muscle may provide an indication of the underlying mechanisms of insulin-related disorders. The aim of this study was to investigate the potential of Fourier transform infrared (FTIR) spectroscopy to reveal the changes in macromolecular composition in weight-bearing and non-weight-bearing muscles of old, obese, insulin-resistant, and young streptozotocin (STZ)-induced diabetic mice. The efficiency of FTIR spectroscopy was evaluated by comparison with the results of gold-standard histochemical techniques. The differences in biomolecular phenotypes and the alterations in muscle composition in relation to their functional properties observed from FTIR spectra suggest that FTIR spectroscopy can detect most of the changes observed in muscle tissue by histochemical analyses and more. Therefore, it could be used as an effective alternative because it allows for the complete characterization of macromolecular composition in a single, relatively simple experiment, avoiding some obvious drawbacks of histochemical methods.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232012498