A bioactivated in vivo assembly nanotechnology fabricated NIR probe for small pancreatic tumor intraoperative imaging

Real-time imaging of the tumour boundary is important during surgery to ensure that sufficient tumour tissue has been removed. However, the current fluorescence probes for bioimaging suffer from poor tumour specificity and narrow application of the imaging window used. Here, we report a bioactivated...

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Bibliographic Details
Published in:Nature communications 2022-01, Vol.13 (1), p.418-13, Article 418
Main Authors: Ren, Han, Zeng, Xiang-Zhong, Zhao, Xiao-Xiao, Hou, Da-yong, Yao, Haodong, Yaseen, Muhammad, Zhao, Lina, Xu, Wan-hai, Wang, Hao, Li, Li-Li
Format: Article
Language:English
Subjects:
631/61/2298
631/61/54/152
631/67/2321
Accumulation
Alanine Transaminase - metabolism
Alkaline Phosphatase - metabolism
Amino Acid Sequence
Animals
Aspartate Aminotransferases - metabolism
Assembly
Biological activity
Blood circulation
Cell Line, Tumor
Female
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent Dyes - toxicity
Fluorescent indicators
Humanities and Social Sciences
Humans
Image enhancement
Intraoperative Care
Liver - enzymology
Medical imaging
Mice
Mice, Inbred BALB C
Mice, Nude
Molecular Dynamics Simulation
multidisciplinary
Nanofibers
Nanofibers - chemistry
Nanotechnology
Optical Imaging
Pancreatic cancer
Pancreatic Neoplasms - diagnostic imaging
Pancreatic Neoplasms - surgery
Peptides - chemistry
Probes
Protein Conformation
Science
Science (multidisciplinary)
Selectivity
Surgery
Tissue Distribution
Toxicity Tests, Acute
Tumors
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Summary:Real-time imaging of the tumour boundary is important during surgery to ensure that sufficient tumour tissue has been removed. However, the current fluorescence probes for bioimaging suffer from poor tumour specificity and narrow application of the imaging window used. Here, we report a bioactivated in vivo assembly (BIVA) nanotechnology, demonstrating a general optical probe with enhanced tumour accumulation and prolonged imaging window. The BIVA probe exhibits active targeting and assembly induced retention effect, which improves selectivity to tumours. The surface specific nanofiber assembly on the tumour surface increases the accumulation of probe at the boundary of the tumor. The blood circulation time of the BIVA probe is prolonged by 110 min compared to idocyanine green. The assembly induced metabolic stability broaden the difference between the tumor and background, obtaining a delayed imaging window between 8–96 h with better signal-to-background contrast (>9 folds). The fabricated BIVA probe permits precise imaging of small sized (
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-27932-y