CBX7 gene expression plays a negative role in adipocyte cell growth and differentiation

We have recently generated knockout mice for the Cbx7 gene, coding for a polycomb group protein that is downregulated in human malignant neoplasias. These mice develop liver and lung adenomas and carcinomas, which confirms a tumour suppressor role for CBX7. The CBX7 ability to downregulate CCNE1 exp...

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Bibliographic Details
Published in:Biology open 2014-09, Vol.3 (9), p.871-879
Main Authors: Forzati, Floriana, Federico, Antonella, Pallante, Pierlorenzo, Colamaio, Marianna, Esposito, Francesco, Sepe, Romina, Gargiulo, Sara, Luciano, Antonio, Arra, Claudio, Palma, Giuseppe, Bon, Giulia, Bucher, Stefania, Falcioni, Rita, Brunetti, Arturo, Battista, Sabrina, Fedele, Monica, Fusco, Alfredo
Format: Article
Language:English
Subjects:
Adipocytes
Adipogenesis
Adipose tissue
Cbx7
Cell differentiation
Differentiation
Embryo cells
Embryo fibroblasts
Fibroblasts
Gene expression
Lung carcinoma
Mouse models
Phenotypes
Polycomb group proteins
Stem cell transplantation
Stem cells
Tumor suppressor genes
Tumors
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Summary:We have recently generated knockout mice for the Cbx7 gene, coding for a polycomb group protein that is downregulated in human malignant neoplasias. These mice develop liver and lung adenomas and carcinomas, which confirms a tumour suppressor role for CBX7. The CBX7 ability to downregulate CCNE1 expression likely accounts for the phenotype of the Cbx7-null mice. Unexpectedly, Cbx7-knockout mice had a higher fat tissue mass than wild-type, suggesting a role of CBX7 in adipogenesis. Consistently, we demonstrate that Cbx7-null mouse embryonic fibroblasts go towards adipocyte differentiation more efficiently than their wild-type counterparts, and this effect is Cbx7 dose-dependent. Similar results were obtained when Cbx7-null embryonic stem cells were induced to differentiate into adipocytes. Conversely, mouse embryonic fibroblasts and human adipose-derived stem cells overexpressing CBX7 show an opposite behaviour. These findings support a negative role of CBX7 in the control of adipocyte cell growth and differentiation.
ISSN:2046-6390
2046-6390
DOI:10.1242/bio.20147872