Luteolin, quercetin, genistein and quercetagetin inhibit the effects of lipopolysaccharide obtained from Porphyromonas gingivalis in H9c2 cardiomyoblasts

• Published in: Cellular & molecular biology letters 2017-09, Vol.22 (1), p.19-19, Article 19
• Main Authors: Gutiérrez-Venegas, Gloria, Torras-Ceballos, Alfredo, Gómez-Mora, Juan Arturo, Fernández-Rojas, Berenice
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Apoptosis; Cardiomyoblasts; Chromones - pharmacology; Cyclooxygenase 2 - drug effects; Cyclooxygenase 2 - genetics; Cyclooxygenase-2; Dental plaque; Endocarditis; Flavonoids; Flavonoids - pharmacology; Fluorides; Gene Expression Regulation; Genistein; Genistein - pharmacology; Gram-negative bacteria; Inflammation; Interleukin 1; Interleukin 6; Kinases; Lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - toxicity; Luteolin - pharmacology; MAP kinase; Mitogen-activated protein kinase; Mitogen-Activated Protein Kinases - antagonists & inhibitors; Mitogen-Activated Protein Kinases - drug effects; Molecular structure; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - enzymology; Myocytes, Cardiac - pathology; Oxidative stress; Periodontitis; Phosphorylation; Porphyromonas gingivalis; Porphyromonas gingivalis - chemistry; Protein kinase C; Proteins; Quercetin; Quercetin - pharmacology; Rats; Sodium; Statistical analysis; Tumor necrosis factor-α; Variance analysis
• ISSN: 1425-8153; 1689-1392
• DOI: 10.1186/s11658-017-0047-z

One of the microorganisms from dental plaque associated with severe inflammatory responses in infectious endocarditis is . It is a Gram-negative bacteria harvested from chronic periodontitis patients. Lipopolysaccharide (LPS) obtained from promotes the expressions of interleukin-1 (IL-1), IL-6 and tumor necrosis factor alpha (TNF-α). Flavonoids are thought to participate in processes that control inflammation, such as the expression of cyclooxygenase-2 (COX-2). We investigated the effects of luteolin, quercetin, genistein and quercetagetin on cardiomyoblasts treated with LPS alone or in combination with following inhibitors p38 (SB203580), ERK (PD98059), JNK (SP600125) and PKC (Calphostin C) for 1 h. The kinase activation and COX-2 expression levels were determined at the gene and protein levels. These flavonoids are considered to inhibit the activation of mitogen-activated protein kinase (MAPK) and the degradation of inhibitor of kappa B-alpha (IκB-α). They also play a role in COX-2 expression. We conclude that the tested flavonoids inhibit inflammatory responses induced by LPS in H9c2 cells.