Cochayuyo (Durvillaea incurvata) Extracts: Their Impact on Starch Breakdown and Antioxidant Activity in Pasta during In Vitro Digestion

• Published in: Foods 2023-09, Vol.12 (18), p.3326
• Main Authors: Pacheco, Luz Verónica, Parada, Javier, Pérez-Correa, José R, Mariotti-Celis, María Salomé, Simirgiotis, Mario
• Format: Article
• Language: English
• Subjects: Algae; anti-diabetic effect; Antioxidants; Archaeology; Calibration; Diabetes; Digestibility; Digestion; Digestive system; edible seaweed; Enzymatic activity; Ethanol; Food; Food science; Gastrointestinal tract; Glucosidase; High performance liquid chromatography; Lipids; Liquid chromatography; Noodles; Phenolic compounds; Phenols; phlorotannin; Polyphenols; Polysaccharides; Proteins; Scientific equipment and supplies industry; Seaweeds; Solvents; Starch; starch digestion; Type 2 diabetes; Vitamin E; α-Glucosidase
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods12183326

Seaweeds, notably cochayuyo (Durvillaea incurvata), are recognized for their rich macro- and micronutrient content, along with their inhibitory effects on the α-glucosidase enzyme. The present study aims to evaluate the effectiveness of this inhibition in actual starchy food products under in vitro gastrointestinal conditions. This study utilized freeze-dried cochayuyo, extracted using hot pressurized liquid extraction with 50% ethanol at 120 °C and 1500 psi. The inhibition mechanism of α-glucosidase was determined, and the polyphenol composition of the extract was analyzed using Ultra-High-Performance Liquid Chromatography. This study further evaluated the extract’s impact on starch digestibility, total phenolic content, and antioxidant capacity in pasta (noodles) as representative starchy food under gastrointestinal conditions. The results indicate that the α-glucosidase inhibition mechanism is of mixed type. Phenolic compounds, primarily tetraphloroethol, could contribute to this anti-enzymatic activity. The extract was observed to decrease starch digestibility, indicated by a lower rate constant (0.0158 vs. 0.0261 min−1) and digested starch at an infinite time (77.4 vs. 80.5 g/100 g). A significant increase (~1200 vs. ~390 µmol TROLOX/100 g) in antioxidant activity was also noted during digestion when the extract was used. Thus, this study suggests that the cochayuyo extract can reduce starch digestion and enhance antioxidant capacity under gastrointestinal conditions.