Epigenome‐wide DNA methylation analysis of myasthenia gravis

Myasthenia gravis (MG) is a common neuromuscular junction disorder and autoimmune disease mediated by several antibodies. Several studies have shown that genetic factors play an important role in MG pathogenesis. To gain insight into the epigenetic factors affecting MG, we report here genome‐scale D...

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Bibliographic Details
Published in:FEBS open bio 2023-07, Vol.13 (7), p.1375-1389
Main Authors: Lin, Jingjing, Tao, Linshuang, Deng, Lu, Zhou, Ruyi, Lou, Shuyue, Chen, Songfang, Chen, Xuanyu, Lu, Chunxing, Li, Peijun, Hu, Beilei
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Autoimmune Diseases
Bioinformatics
Biomarkers
Biotechnology
Cell culture
Cell lines
Demethylation
DNA methylation
DNA Methylation - genetics
Down-regulation
Epigenetics
Epigenome
Gene expression
Genetic factors
Genomes
high throughput
Hospitals
Kinases
Leukocytes
Life sciences
Medical research
Methylation
Mice
Musculoskeletal System
Myasthenia gravis
Myasthenia Gravis - genetics
Myasthenia Gravis - therapy
neuromuscular junction
Neuromuscular junctions
Ontology
Pathogenesis
Phosphatase
Proteins
RNA, Messenger - genetics
Yes-associated protein
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Summary:Myasthenia gravis (MG) is a common neuromuscular junction disorder and autoimmune disease mediated by several antibodies. Several studies have shown that genetic factors play an important role in MG pathogenesis. To gain insight into the epigenetic factors affecting MG, we report here genome‐scale DNA methylation profiles of MG. DNA was extracted from eight MG patients and four healthy controls for genome‐wide DNA methylation analysis using the Illumina HumanMethylation 850K BeadChip. Verification of pyrosequencing was conducted based on differential methylation positions. Subsequently, C2C12 and HT22 cell lines (derived from mouse) were treated with demethylation drugs. Transcribed mRNA of the screened differential genes was detected using quantitative real‐time PCR. The control and MG group were compared, and two key probe positions were selected. The corresponding genes were CAMK1D and CREB5 (P 
ISSN:2211-5463
2211-5463
DOI:10.1002/2211-5463.13656