The Influence of Solid and Liquid Systems In Vitro on the Growth and Biosynthetic Characteristics of Microshoot Culture of Spiraea betulifolia ssp. aemiliana

• Published in: International journal of molecular sciences 2023-01, Vol.24 (3), p.2362
• Main Authors: Zheleznichenko, Tatiana V, Muraseva, Dinara S, Erst, Andrey S, Kuznetsov, Alexander A, Kulikovskiy, Maxim S, Kostikova, Vera A
• Format: Article
• Language: English
• Subjects: Antioxidants - pharmacology; Biological activity; Biomass; Biosynthesis; Carboxylic Acids; Chromatography, High Pressure Liquid; Comparative analysis; flavonoid; Flavonoids; Flavonoids - pharmacology; Flowers & plants; Glycosides; Herbal medicine; High performance liquid chromatography; in vitro culture; Kaempferol; Kaempferols - analysis; liquid culture medium; Metabolites; phenol carboxylic acid; Phenolic compounds; Phenols; Phenols - analysis; Plant Extracts - pharmacology; Scavenging; solid culture medium; Spiraea; Spiraea betulifolia; Spiraea betulifolia ssp. aemiliana
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24032362

The paper focuses on the growth dynamics and biosynthetic characteristics of the microshoot culture of ssp. obtained in vitro in agar-solidified and liquid media. Microshoots cultured in either type of media showed similar growth dynamics. The most active culture growth was observed from day 35 to day 60. A comparative analysis of the contents of flavonoids and phenol carboxylic acids showed a higher level of phenol carboxylic acids (5.3-6.84%) and a stronger 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity (half-maximal inhibitory concentration: 341 µg/mL) in ssp. microshoots grown in the liquid medium compared to the microshoots cultured in the solid medium. The flavonoid content of the cultured microshoot did not depend on the consistency of the medium. High-performance liquid chromatography (HPLC) was employed to study the profile and levels of phenolic compounds in microshoots, intact plants, and ex vitro-acclimated ssp. plants. The concentration of kaempferol glycosides was found to be higher in microshoots (1.33% in the solid medium, 1.06% in the liquid medium) compared to intact plants and ex vitro-acclimated plants. Thus, the microshoots of ssp. cultured in the liquid medium rapidly increase their biomass and are an inexpensive promising source of biologically active antioxidant substances, mainly phenol carboxylic acids and kaempferol glycosides.