Antioxidant and Antibacterial Assays on Polygonum minus Extracts: Different Extraction Methods

The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity of Polygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated us...

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Bibliographic Details
Published in:International Journal of Chemical Engineering 2015-01, Vol.2015 (2015), p.1-10
Main Authors: Baharum, Syarul Nataqain, Dewi, Kurnia Harlina, Anuar, Nurina, Markom, Masturah, Hassim, Norsyamimi, Noor, Normah Mohd
Format: Article
Language:English
Subjects:
Aldehydes
Antiinfectives and antibacterials
Antioxidants
Assaying
Bacillus subtilis
Bioassays
Biological activity
Carbon dioxide
Escherichia coli
Ethanol
Free radicals
Inhibition
Methods
Methyl alcohol
Molecular weight
Polygonum
Solvent extraction
Solvents
Staphylococcus aureus
Studies
Temperature effects
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Summary:The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity of Polygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest polyphenol content was obtained from the medium polarity methanol extract of the leaf portion (645.60 ± 166.68 gallic acid equivalents/100 g (GAEs/100 g)). It also showed the highest antioxidant power for FRAP and DPPH radical inhibition and exhibited the largest inhibition zone in antibacterial activity on Bacillus subtilis (Gram+), Staphylococcus aureus (Gram+), and Escherichia coli (Gram−). The phase behavior and aldehyde profiles were further investigated using SFE with different cosolvents. The results indicated that a 50% ethanol-water cosolvent yielded the best aldehyde profiles in the presence of decanal, undecanal, and dodecanal.
ISSN:1687-806X
1687-8078
DOI:10.1155/2015/826709