Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose

Butanol is a second generation biofuel produced by Clostridium acetobutylicum through acetone-butanol-ethanol (ABE) fermentation process. Shotgun proteomics provides a direct approach to study the whole proteome of an organism in depth. This paper focuses on shotgun proteomic profiling of C. acetobu...

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Bibliographic Details
Published in:Proteome science 2011-10, Vol.9 (1), p.66-66, Article 66
Main Authors: Sivagnanam, Kumaran, Raghavan, Vijaya Gs, Shah, Manesh, Hettich, Robert L, Verberkmoes, Nathan C, Lefsrud, Mark G
Format: Article
Language:English
Subjects:
09 BIOMASS FUELS
ABE fermentation
ACETONE
Bacteria
BASIC BIOLOGICAL SCIENCES
BIOFUELS
Butanol
BUTANOLS
CHROMATOGRAPHY
Clostridium
CLOSTRIDIUM ACETOBUTYLICUM
Dextrose
ETHANOL
FERMENTATION
FUNCTIONALS
GLUCOSE
Liquid chromatography
mass spectrometry
MASS SPECTROSCOPY
Microbiology
Physiological aspects
PRODUCTION
PROTEINS
shotgun proteomics
Studies
SUBSTRATES
XYLOSE
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Summary:Butanol is a second generation biofuel produced by Clostridium acetobutylicum through acetone-butanol-ethanol (ABE) fermentation process. Shotgun proteomics provides a direct approach to study the whole proteome of an organism in depth. This paper focuses on shotgun proteomic profiling of C. acetobutylicum from ABE fermentation using glucose and xylose to understand the functional mechanisms of C. acetobutylicum proteins involved in butanol production. We identified 894 different proteins in C. acetobutylicum from ABE fermentation process by two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS) method. This includes 717 proteins from glucose and 826 proteins from the xylose substrate. A total of 649 proteins were found to be common and 22 significantly differentially expressed proteins were identified between glucose and xylose substrates. Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C. acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report.
ISSN:1477-5956
1477-5956
DOI:10.1186/1477-5956-9-66