Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. The...

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Bibliographic Details
Published in:Czech Journal of Food Sciences 2013-01, Vol.31 (6), p.627-632
Main Authors: Nagel, Orlando G., Molina, Maria Pilar, Althaus, Rafael L.
Format: Article
Language:English
Subjects:
b. subtilis
bioassay
Bioassays
Culture media
Design of experiments
Experimental design
logistic regression model
microbial inhibition method
Microorganisms
Regression analysis
Regression models
Response time
Sulfonamides
Trimethoprim
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Summary:We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (24-1). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.
ISSN:1212-1800
1805-9317
DOI:10.17221/494/2011-CJFS