Inhibitory Effects of Pinostilbene on Adipogenesis in 3T3-L1 Adipocytes: A Study of Possible Mechanisms

• Published in: International journal of molecular sciences 2021-12, Vol.22 (24), p.13446
• Main Authors: Chung, You Chul, Hyun, Chang-Gu
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Accumulation; Adipocytes; Adipocytes - metabolism; Adipogenesis; Adipogenesis - drug effects; AKT; AKT protein; AMP-Activated Protein Kinases - metabolism; Animals; Bioavailability; Biocompatibility; Biological activity; Body fat; Cancer; Cell activation; Colon; Cyclic AMP response element-binding protein; Cytotoxicity; Energy; Extracellular Signal-Regulated MAP Kinases - metabolism; Fatty acids; Fatty-acid synthase; Homeostasis; Hyperplasia; Insulin; Kinases; Lipids; MAP kinase; MAP Kinase Signaling System - drug effects; MAPK; Metabolism; Metabolites; Mice; Microbiota; Neuroprotection; Obesity; Peroxisome proliferator-activated receptors; Phosphorylation; Phosphorylation - drug effects; pinostilbene; Proteins; Resveratrol; Signal transduction; Sterol regulatory element-binding protein; Stilbenes - pharmacology; Structural stability; Transcription factors; Transcription Factors - metabolism; Triglycerides; Ultraviolet radiation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222413446

Resveratrol is a phytoalexin with multiple bioactive properties, including antioxidative, neuroprotective, cardioprotective, and anticancer effects. However, resveratrol exhibits structural instability in response to UV irradiation, alkaline pH, and oxygen exposure. Thus, resveratrol derivatives have attracted considerable research interest. In this study, we aimed to evaluate the anti-adipogenic effects of pinostilbene hydrate (PH), a methylated resveratrol derivative, in 3T3-L1 cells. We also evaluated the mechanisms underlying the effects of PH on adipogenesis in 3T3-L1 adipocytes. Oil Red O staining, lipid accumulation assay, and triglyceride (TG) content assay revealed that PH significantly inhibited lipid and TG accumulation without cytotoxicity. In addition, we determined that PH decreased the expression of adipogenesis-related transcription factors, such as PPARγ, C/EBPα, SREBP-1c, and FABP4, and the phosphorylation of MAPK and protein kinase B (AKT). Moreover, PH attenuated the expression of CREB and C/EBPβ, while increasing the phosphorylation of AMPK and ACC, and decreasing the expression of fatty acid synthase and FABP4. Based on these results, we suggest that PH suppresses adipogenesis in 3T3-L1 cells via the activation of the AMPK signaling pathway and the inhibition of the MAPK and AKT insulin-dependent signaling pathways.