Cardioprotective Mechanisms of Interrupted Anesthetic Preconditioning with Sevoflurane in the Setting of Ischemia/Reperfusion Injury in Rats

Background: Anesthetic preconditioning (AP) is known to mimic ischemic preconditioning. The purpose of this study was to investigate the effects of an interrupted sevoflurane administration protocol on myocardial ischemia/reperfusion (I/R) injury. Methods: Male Wistar rats (n = 60) were ventilated f...

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Published in:Applied sciences 2022-02, Vol.12 (3), p.1476
Main Authors: Popescu, Mihaela Roxana, Pavel, Bogdan, Isvoranu, Gheorghita, Ceafalan, Laura Cristina, Panaitescu, Anca Maria, Sava, Ruxandra Irina, Vlad, Adelina, Zagrean, Leon
Format: Article
Language:English
Subjects:
Alveoli
Anesthesia
anesthetic preconditioning
Antibodies
Apoptosis
c-Kit protein
Cardiovascular diseases
Cytokines
Fibrosis
Granulocytes
Health risks
Ischemia
ischemia/reperfusion injury
Laboratory animals
Myocardial ischemia
myocardial protection
Ostomy
Preconditioning
Reperfusion
Sevoflurane
Statistical analysis
Vascular endothelial growth factor
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Summary:Background: Anesthetic preconditioning (AP) is known to mimic ischemic preconditioning. The purpose of this study was to investigate the effects of an interrupted sevoflurane administration protocol on myocardial ischemia/reperfusion (I/R) injury. Methods: Male Wistar rats (n = 60) were ventilated for 30 min with room air (control group, CG) or with a mixture of air and sevoflurane (1 minimum alveolar concentration—MAC) in 5-min cycles, alternating with 5-min wash-out periods (preconditioned groups). Cytokines implicated in the AP response were measured. An (I/R) lesion was produced immediately after the sham intervention (CG) and preconditioning protocol (early AP group, EAPG) or 24 h after the intervention (late AP group, LAPG). The area of fibrosis, the degree of apoptosis and the number of c-kit+ cells was estimated for each group. Results: Cytokine levels were increased post AP. The area of fibrosis decreased in both EAPG and LAPG compared to the CG (p < 0.0001). When compared to the CG, the degree of apoptosis was reduced in both LAPG (p = 0.006) and EAPG (p = 0.007) and the number of c-kit+ cells was the greatest for the LAPG (p < 0.0001). Conclusions: Sevoflurane preconditioning, using an interrupted anesthesia protocol, is efficient in myocardial protection and could be beneficial to reduce perioperative or periprocedural ischemia in patients with increased cardiovascular risk.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12031476