Wound healing and antimicrobial activity of two classical formulations of Laghupanchamula in rats

Wounds affect a large number of patients and seriously reduce the quality of life. The wound as a medical problem was first discussed by Maharshi Agnivesha in Agnivesha Samhita (later known as Charaka Samhita) as Vrana. Laghupanchamula denotes a combination of the roots of five herbs. However, in Ay...

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Bibliographic Details
Published in:Journal of ayurveda and integrative medicine 2015-10, Vol.6 (4), p.241-247
Main Authors: Ghildiyal, Shivani, Gautam, Manish K, Joshi, Vinod K, Goel, Raj K
Format: Article
Language:English
Subjects:
Albinism
Analgesics
Anti-infective agents
Antimicrobial
Antimicrobial agents
Ayurvedic medicine
Bacteria
Collagen
Conflicts of interest
Drug dosages
excision wound
Fibroblasts
Flavonoids
Health aspects
incision wound
Inflammation
Laboratory animals
Laghupanchamula
minimum inhibitory concentration
Original
Seeds
Vitamin E
Wound healing
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Summary:Wounds affect a large number of patients and seriously reduce the quality of life. The wound as a medical problem was first discussed by Maharshi Agnivesha in Agnivesha Samhita (later known as Charaka Samhita) as Vrana. Laghupanchamula denotes a combination of the roots of five herbs. However, in Ayurvedic classics, besides four common herbs viz. Kantakari, Brihati, Shalaparni and Prinshniparni, the fifth one is either Gokshura (LPG) or Eranda (LPE), and both formulations have been documented to have wound healing (Vrana) activity. The present study was undertaken to determine the in vivo wound healing activity and in vitro antimicrobial activity of 50% ethanolic extract of Laghupanchamula containing Gokshura (LPGE) and Laghupanchamula containing Eranda (LPEE) in rats with acute toxicity in mice. LPGE and LPEE (1000 mg/kg) was administered orally, once daily for 10 days (incision wound model) or for 24 days (excision wound model) in rats. LPGE and LPEE was studied for its in vitro antimicrobial and in vivo wound breaking strength (WBS) (incision model) and rate of contraction, period of epithelization and histology of skin (excision model). LPGE and LPEE showed antimicrobial activity against skin pathogens, enhanced WBS, rate of contraction, skin collagen tissue formation and early epithelization period with low scar area indicating enhanced healing with histological evidence of more collagen formation in skin tissues. LPGE and LPEE also showed anti-bacterial activity and seemed to be safe, and use of both formulations in Laghupanchamula for their wound healing and anti-microbial activities is thus authenticated.
ISSN:0975-9476
0976-2809
DOI:10.4103/0975-9476.157952