Antioxidant Potential of Sulfated Polysaccharides from Padina boryana ; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Elevated levels of reactive oxygen species (ROS) damage the internal cell components. , a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of were assessed against hydrogen peroxide (H O ) induced c...

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Bibliographic Details
Published in:Marine drugs 2020-04, Vol.18 (4), p.212
Main Authors: Jayawardena, Thilina U, Wang, Lei, Sanjeewa, K K Asanka, Kang, Sang In, Lee, Jung-Suck, Jeon, You-Jin
Format: Article
Language:English
Subjects:
Algae
Animals
antioxidant
Antioxidants
Antioxidants - chemistry
Antioxidants - pharmacology
Apoptosis
Catalase
Damage
Enzymes
Ethanol
Extracellular
Fish
Fourier transforms
Freshwater fishes
Fucoidan
Hydrogen peroxide
Industrial applications
Lipid peroxidation
Lipids
Maldives
Metabolites
Models, Animal
Oxidative stress
Oxidative Stress - drug effects
Padina boryana
Peroxidation
Phaeophyceae
Physiology
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - pharmacology
Pretreatment
Proteins
Reactive oxygen species
Saccharides
Scavenging
Signal transduction
sulfated polysaccharide
Superoxide dismutase
Zebrafish
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Summary:Elevated levels of reactive oxygen species (ROS) damage the internal cell components. , a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of were assessed against hydrogen peroxide (H O ) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H O . PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs' ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.
ISSN:1660-3397
1660-3397
DOI:10.3390/md18040212