Evaluation of the automated system Vitek2 for identification and antimicrobial susceptibility testing of Brazilian Gram-positive cocci strains

Automated instruments offer many advantages for clinical laboratories. Nevertheless, they can have problems identifying and determining susceptibilities of some pathogens. Vitek2 (bioMérieux) is an automated system that was recently introduced to Brazil. We evaluated the performance of this equipmen...

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Bibliographic Details
Published in:The Brazilian journal of infectious diseases 2009-04, Vol.13 (2), p.107-110
Main Authors: d'Azevedo, Pedro Alves, Siquiera, Itacy, Gugel, Juliana, Antunes, Ana Lúcia Souza, Secchi, Carina, Pasternak, Jacyr, Martino, Marinês Dalla Vale
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
antimicrobial susceptibility testing
Automated system
Automatic data collection systems
Automation, Laboratory - methods
Cross infection
Disease susceptibility
Drug resistance
Drug therapy
Enterococcal infections
Enterococcus - classification
Enterococcus - drug effects
Gram-positive cocci
Health aspects
Humans
identification
INFECTIOUS DISEASES
Microbial Sensitivity Tests - instrumentation
Nosocomial infections
Oxacillin
Patient outcomes
Reproducibility of Results
Risk factors
Staphylococcus - classification
Staphylococcus - drug effects
Vitek2
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Summary:Automated instruments offer many advantages for clinical laboratories. Nevertheless, they can have problems identifying and determining susceptibilities of some pathogens. Vitek2 (bioMérieux) is an automated system that was recently introduced to Brazil. We evaluated the performance of this equipment for Brazilian isolates that had been characterized using reference identification and antimicrobial susceptibility testing methods. Ninety-nine strains of Gram-positive cocci from a local reference center collection were analyzed, consisting of 50 coagulase-negative Staphylococcus (CoNS) and 49 Enterococcus and related species. Vitek2 correctly identified 79.8% (79/99) of the isolates. Oxacillin resistance was detected in 76% (19/25) of resistant S. epidermidis strains and in 88% (22/25) of other resistant CoNS species strains. Vancomycin resistance was detected in 100% (20/20) of resistant Enterococcus and related species strains. Vitek2 performed very well for the identification of S. epidermidis and non-epidermidis staphylococci, and for the detection of vancomycin resistance in Enterococcus and related species. However, the system needs improvement in order to provide reliable results for the characterization of some CoNS species, identification of Enterococcus and related species and for detecting oxacillin resistance in CoNS.
ISSN:1678-4391
1413-8670
1678-4391
DOI:10.1590/S1413-86702009000200007