Circadian rhythm of intracellular protein synthesis signaling in rat cardiac and skeletal muscles

Intracellular signaling exhibits circadian variation in the suprachiasmatic nucleus and liver. However, it is unclear whether circadian regulation also extends to intracellular signaling pathways in the cardiac and skeletal muscles. Here, we examined circadian variation in the intracellular mammalia...

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Bibliographic Details
Published in:Biochemistry and biophysics reports 2017-03, Vol.9 (C), p.153-158
Main Authors: Chang, Shuo-Wen, Yoshihara, Toshinori, Machida, Shuichi, Naito, Hisashi
Format: Article
Language:English
Subjects:
70 kDa ribosomal protein S6 kinase 1
Circadian variation
Extracellular signal-regulated kinase 1/2
Heart
Mammalian target of rapamycin
Skeletal muscle
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Summary:Intracellular signaling exhibits circadian variation in the suprachiasmatic nucleus and liver. However, it is unclear whether circadian regulation also extends to intracellular signaling pathways in the cardiac and skeletal muscles. Here, we examined circadian variation in the intracellular mammalian target of rapamycin (mTOR)/70 kDa ribosomal protein S6 kinase 1 (p70S6K) and extracellular signal-regulated kinase (ERK) pathways, which regulate protein synthesis in rat cardiac and skeletal muscles. Seven-week-old male Wistar rats were assigned to six groups: Zeitgeber time (ZT) 2, ZT6, ZT10, ZT14, ZT18, and ZT22 (ZT0, lights on; ZT12, lights off). The cardiac, plantaris, and soleus muscles were removed after a 12-h fasting period, and signal transducers involved in protein synthesis (mTOR, p70S6K, and ERK) were analyzed by western blotting. Circadian rhythms of signal transducers were observed in both cardiac (mTOR, p70S6K, and ERK) and plantaris (p70S6K and ERK) muscles (
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2016.12.005