Study of class 1 integrons in multidrug-resistant uropathogenic Escherichia coli isolated from different hospitals in Karachi

• Published in: Iranian journal of basic medical sciences 2018-10, Vol.21 (10), p.1079-1082
• Main Authors: Zeeshan Khan, Fouzia, Nawaz, Tehseen, Mirani, Zulfiqar Ali, Khan, Saeed, Raza, Yasir, Kazmi, Shahana Urooj
• Format: Article
• Language: English
• Subjects: Antibiotics; Class 1 integrons; Drug resistance; E coli; ESBLs; MDR E. coli; Multidrug resistance; Multidrug resistant organisms; Original; Uropathogenic E. coli
• ISSN: 2008-3866; 2008-3874
• DOI: 10.22038/IJBMS.2018.28807.6966

is the key pathogen in the family producing ESBL (extended spectrum β-lactamase) and associated with community-acquired infections. Therefore, this study was planned to determine the antibiotic susceptibility pattern of uropathogenic , prevalence of the ESBL gene group and class 1 integrons. Clinical isolates of uropathogenic were isolated from different hospitals of Karachi. Antibiotic susceptibility test was performed by Kirby-Bauer Methods. Presence of β- lactamases genes (CTX, TEM, and SHV) and integron 1 were identified by polymerase chain reaction (PCR). Out of 500, 105 isolates were identified as multi-drug resistant (MDR) uropathogenic . The subject MDR isolates showed the highest resistance to aztreonam, amoxil/ clavulanic acid, ampicillin, cotrimoxazole, ceftriaxone, cefipime, and cefuroxime. Genetic analysis showed that the majority of the MDR carry CTX M1 (57.1%) followed by TEM (33.3%) and SHV (9.5%). Moreover, 79% of MDR harbored class 1 integrons, whereas all three conserved genes for class 1 integrons were present in 58% of MDR . This study is helpful to provide information regarding the antibiotic susceptibility pattern, distribution ESBLs and class 1 integrons among uropathogenic .