Oxidative Stress and the ER Stress Response in a Murine Model for Early-Stage Alcoholic Liver Disease

Alcoholic liver disease (ALD) is a primary cause of morbidity and mortality in the United States and constitutes a significant socioeconomic burden. Previous work has implicated oxidative stress and endoplasmic reticulum (ER) stress in the etiology of ALD; however, the complex and interrelated natur...

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Bibliographic Details
Published in:Journal of Toxicology 2012-01, Vol.2012 (2012), p.129-140
Main Authors: Orlicky, David J., MacLean, Kenneth N., Petersen, Dennis R., Galligan, James J., Franklin, Christopher C., Jiang, Hua, Backos, Donald S., Smathers, Rebecca L., Shearn, Colin T., Fritz, Kristofer S.
Format: Article
Language:English
Subjects:
Diet
Disease
Enzymes
Ethanol
Lipids
Liver cirrhosis
Liver diseases
Oxidative stress
Protein folding
Sterols
Stress response
Studies
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Summary:Alcoholic liver disease (ALD) is a primary cause of morbidity and mortality in the United States and constitutes a significant socioeconomic burden. Previous work has implicated oxidative stress and endoplasmic reticulum (ER) stress in the etiology of ALD; however, the complex and interrelated nature of these cellular responses presently confounds our understanding of ethanol-induced hepatopathy. In this paper, we assessed the pathological contribution of oxidative stress and ER stress in a time-course mouse model of early-stage ALD. Ethanol-treated mice exhibited significant hepatic panlobular steatosis and elevated plasma ALT values compared to isocaloric controls. Oxidative stress was observed in the ethanol-treated animals through a significant increase in hepatic TBARS and immunohistochemical staining of 4-HNE-modified proteins. Hepatic glutathione (GSH) levels were significantly decreased as a consequence of decreased CBS activity, increased GSH utilization, and increased protein glutathionylation. At the same time, immunoblot analysis of the PERK, IRE1α, ATF6, and SREBP pathways reveals no significant role for these UPR pathways in the etiology of hepatic steatosis associated with early-stage ALD. Collectively, our results indicate a primary pathogenic role for oxidative stress in the early initiating stages of ALD that precedes the involvement of the ER stress response.
ISSN:1687-8191
1687-8205
DOI:10.1155/2012/207594