Synergistic Effect of Vitamin C with Cisplatin for Inhibiting Proliferation of Gastric Cancer Cells

Ascorbic acid, known as vitamin C, has been used in combination with a number of cytotoxic agents in vitro and in vivo with contradictory results on its effectiveness. It is believed that vitamin C can sensitize different cancer cells to common therapy strategies such as chemotherapy and radiotherap...

Full description

Saved in:
Bibliographic Details
Published in:Iranian biomedical journal 2020-03, Vol.24 (2), p.119-127
Main Authors: Ghavami, Ghazaleh, Sardari, Soroush
Format: Article
Language:English
Subjects:
Apoptosis
Ascorbic acid
Biocompatibility
Cancer
Cancer therapies
Cell cycle
Cell proliferation
Chemotherapy
Cisplatin
Cytotoxic agents
Cytotoxicity
Flow cytometry
Full Length
Gastric cancer
Necrosis
neoplasms
Radiation therapy
Synergistic effect
Toxicity
Vitamin C
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ascorbic acid, known as vitamin C, has been used in combination with a number of cytotoxic agents in vitro and in vivo with contradictory results on its effectiveness. It is believed that vitamin C can sensitize different cancer cells to common therapy strategies such as chemotherapy and radiotherapy. During current research, the combination effect of vitamin C with cisplatin was evaluated against gastric cancer cells. MTT-based proliferation assay, combination index method, and flow cytometry technique were employed for the assessment of cell cycle and determination of apoptosis/necrosis on the AGS cell line. Co-treatment of gastric cancer cells with vitamin C in its IC50 dose in addition to cisplatin in both IC50 (10 µg/ml) and five times less (2 µg/ml) doses could increase the cytotoxicity effect of cisplatin in a synergistic manner. Moreover, the pointed co-treatment approach could induce the cell count in sub-G0 phase while reducing it in the G0/G1, G2/M, and S phases. Further findings showed that the combined dose of vitamin C and cisplatin could increase the percentage of apoptotic and necrotic cells in comparison with a single dose of cisplatin. This study introduces a possible approach for the treatment of gastric cancer with more potency and less amount of administered cisplatin to induce toxicity.
ISSN:1028-852X
2008-823X
DOI:10.29252/ibj.24.2.119