Biodegradation of Aflatoxin B1 in Maize Grains and Suppression of Its Biosynthesis-Related Genes Using Endophytic Trichoderma harzianum AYM3

Aflatoxin B1 is one of the most deleterious types of mycotoxins. The application of an endophytic fungus for biodegradation or biosuppression of AFB1 production by was investigated. About 10 endophytic fungal species, isolated from healthy maize plants, were screened for their in vitro AFs-degrading...

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Bibliographic Details
Published in:Journal of fungi (Basel) 2023-02, Vol.9 (2), p.209
Main Authors: Madbouly, Adel K, Rashad, Younes M, Ibrahim, Mohamed I M, Elazab, Nahla T
Format: Article
Language:English
Subjects:
Acetic acid
Aflatoxin B1
biocontrol
Biodegradation
Biosynthesis
Corn
Crops
Cytotoxicity
detoxification
endophyte
Endophytes
Fungi
Fungicides
Infections
Laboratories
Metabolites
Microorganisms
mycotoxins
Natural products
Pathogens
Pesticides
Plant resistance
Propyl acetate
storage
Trichoderma harzianum
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Summary:Aflatoxin B1 is one of the most deleterious types of mycotoxins. The application of an endophytic fungus for biodegradation or biosuppression of AFB1 production by was investigated. About 10 endophytic fungal species, isolated from healthy maize plants, were screened for their in vitro AFs-degrading activity using coumarin medium. The highest degradation potential was recorded for sp. (76.8%). This endophyte was identified using the rDNA-ITS sequence as AYM3 and assigned an accession no. of ON203053. It caused a 65% inhibition in the growth of AYM2 in vitro. HPLC analysis revealed that AYM3 had a biodegradation potential against AFB1. Co-culturing of AYM3 and AYM2 on maize grains led to a significant suppression (67%) in AFB1 production. GC-MS analysis identified two AFB1-suppressing compounds, acetic acid and n-propyl acetate. Investigating effect on the transcriptional expression of five AFB1 biosynthesis-related genes in AYM2 revealed the downregulating effects of AYM3 metabolites on expression of and genes. Using HepaRG cell line, the cytotoxicity assay indicated that AYM3 metabolites were safe. Based on these results, it can be concluded that AYM3 may be used to suppress AFB1 production in maize grains.
ISSN:2309-608X
2309-608X
DOI:10.3390/jof9020209