Protective Effects of Laminaria japonica Polysaccharide Composite Microcapsules on the Survival of Lactobacillus plantarum during Simulated Gastrointestinal Digestion and Heat Treatment

• Published in: Marine drugs 2024-06, Vol.22 (7), p.308
• Main Authors: Guo, Honghui, Zhou, Yelin, Xie, Quanling, Chen, Hui, Zhang, Ming'en, Yu, Lei, Yan, Guangyu, Chen, Yan, Lin, Xueliang, Zhang, Yiping, Hong, Zhuan
• Format: Article
• Language: English
• Subjects: Acids; Bacteria; Calorimetry; Capsules; Cell survival; cell viability; Denaturation; Differential scanning calorimetry; Digestion; Digestion - drug effects; Digestive system; Disintegration; Edible Seaweeds; Enzymes; gastric juice; gastrointestinal digestion; gastrointestinal system; Gastrointestinal tract; Gastrointestinal Tract - drug effects; Gastrointestinal Tract - microbiology; Gelatin; Gelatin - chemistry; Gelatin - pharmacology; Heat; Heat treatment; Heat treatments; Hot Temperature; Hydrogen bonds; Hydrogen-Ion Concentration; in vitro digestion; intestinal secretions; L-Lactate dehydrogenase; Lactate; Lactate dehydrogenase; Lactobacilli; Lactobacillus plantarum; Laminaria - chemistry; Laminaria japonica; Laminaria japonica polysaccharide; Microbial Viability - drug effects; microcapsule; Microcapsules; Microencapsulation; Microstructure; Morphology; Na+/K+-exchanging ATPase; Particle size; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Probiotics; Probiotics - administration & dosage; Probiotics - pharmacology; Proteins; Saccharina japonica; Sodium; sodium caseinate; spray drying; Survivability; Survival; survival rate; Thermal energy; Thermal stability; Thermogravimetry; Vibration; Zeta potential
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md22070308

To improve probiotics' survivability during gastrointestinal digestion and heat treatment, was microencapsulated by spray-drying using polysaccharide/sodium caseinate/gelatin (LJP/SC/GE) composites. Thermogravimetry and differential scanning calorimetry results revealed that the denaturation of LJP/SC/GE microcapsules requires higher thermal energy than that of SC/GE microcapsules, and the addition of LJP may improve thermal stability. Zeta potential measurements indicated that, at low pH of the gastric fluid, the negatively charged LJP attracted the positively charged SC/GE, helping to maintain an intact microstructure without disintegration. The encapsulation efficiency of -loaded LJP/SC/GE microcapsules reached about 93.4%, and the survival rate was 46.9% in simulated gastric fluid (SGF) for 2 h and 96.0% in simulated intestinal fluid (SIF) for 2 h. In vitro release experiments showed that the LJP/SC/GE microcapsules could protect the viability of in SGF and release probiotics slowly in SIF. The cell survival of LJP/SC/GE microcapsules was significantly improved during the heat treatment compared to SC/GE microcapsules and free cells. LJP/SC/GE microcapsules can increase the survival of by maintaining the lactate dehydrogenase and Na -K -ATPase activity. Overall, this study demonstrates the great potential of LJP/SC/GE microcapsules to protect and deliver probiotics in food and pharmaceutical systems.