Evolution of Target-Site Resistance to Glyphosate in an Amaranthus palmeri Population from Argentina and Its Expression at Different Plant Growth Temperatures

• Published in: Plants (Basel) 2019-11, Vol.8 (11), p.512
• Main Authors: Kaundun, Shiv Shankhar, Jackson, Lucy Victoria, Hutchings, Sarah-Jane, Galloway, Jonathan, Marchegiani, Elisabetta, Howell, Anushka, Carlin, Ryan, Mcindoe, Eddie, Tuesca, Daniel, Moreno, Raul
• Format: Article
• Language: English
• Subjects: Amaranthus palmeri; argentina; epsps over-expression; Glyphosate; Herbicide resistance; Herbicides; Meristems; Mutation; Night; Overexpression; p106s epsps mutation; Plant growth; Population; Proline; resistance mechanism; Seeds; Serine; Soybeans; Translocation
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants8110512

The mechanism and expression of resistance to glyphosate at different plant growing temperatures was investigated in an population (VM1) from a soybean field in Vicuña Mackenna, Cordoba, Argentina. Resistance was not due to reduced glyphosate translocation to the meristem or to duplication, as reported for most US samples. In contrast, a proline 106 to serine target-site mutation acting additively with over-expression (1.8-fold increase) was respectively a major and minor contributor to glyphosate resistance in VM1. Resistance indices based on LD values generated using progenies from a cross between 52 PS106 VM1 individuals were estimated at 7.1 for homozygous SS106 and 4.3 for heterozygous PS106 compared with homozygous wild PP106 plants grown at a medium temperature of 24 °C day/18 °C night. A larger proportion of wild and mutant progenies survived a single commonly employed glyphosate rate when maintained at 30 °C day/26 °C night compared with 20 °C day/16 night in a subsequent experiment. Interestingly, the P106S mutation was not identified in any of the 920 plants analysed from 115 US populations, thereby potentially reflecting the difference in control practices in Argentina and USA.