Peroxisomes form intralumenal vesicles with roles in fatty acid catabolism and protein compartmentalization in Arabidopsis

• Published in: Nature communications 2020-12, Vol.11 (1), p.6221-13, Article 6221
• Main Authors: Wright, Zachary J., Bartel, Bonnie
• Format: Article
• Language: English
• Subjects: 14; 14/19; 14/35; 42/44; 631/449/448/2024; 631/80/642/2013; 64; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Breakdown; Catabolism; Cell Compartmentation; Complexity; Endoplasmic Reticulum - metabolism; Endosomal Sorting Complexes Required for Transport - metabolism; Endosomes - metabolism; Epidermal Cells - metabolism; Fats; Fatty acids; Fatty Acids - metabolism; Humanities and Social Sciences; Intracellular Membranes - metabolism; Lipids; Membrane proteins; Membrane structure; Membrane structures; Membrane vesicles; Membranes; Metabolism; Metabolites; Microscopy, Confocal; multidisciplinary; Organelles; Oxidation; Peroxisomes; Peroxisomes - metabolism; Plants, Genetically Modified; Protein Transport; Proteins; Science; Science (multidisciplinary); Seedlings - cytology; Seedlings - metabolism; Time-Lapse Imaging - methods; Vesicles; Yeasts
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-20099-y

Peroxisomes are vital organelles that compartmentalize critical metabolic reactions, such as the breakdown of fats, in eukaryotic cells. Although peroxisomes typically are considered to consist of a single membrane enclosing a protein lumen, more complex peroxisomal membrane structure has occasionally been observed in yeast, mammals, and plants. However, technical challenges have limited the recognition and understanding of this complexity. Here we exploit the unusually large size of Arabidopsis peroxisomes to demonstrate that peroxisomes have extensive internal membranes. These internal vesicles accumulate over time, use ESCRT (endosomal sorting complexes required for transport) machinery for formation, and appear to derive from the outer peroxisomal membrane. Moreover, these vesicles can harbor distinct proteins and do not form normally when fatty acid β-oxidation, a core function of peroxisomes, is impaired. Our findings suggest a mechanism for lipid mobilization that circumvents challenges in processing insoluble metabolites. This revision of the classical view of peroxisomes as single-membrane organelles has implications for all aspects of peroxisome biogenesis and function and may help address fundamental questions in peroxisome evolution. Peroxisomes are organelles compartmentalising metabolic reactions such as the breakdown of fats, and are commonly thought of as single membrane-bound compartments. Here the authors show that Arabidopsis peroxisomes contain extensive internal vesicles that form from the bounding membrane in an ESCRT-dependent process.