Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry

An ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-...

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Bibliographic Details
Published in:E3S web of conferences 2020-01, Vol.213, p.1033
Main Authors: Zha, Yu-bing, Yang, Chun-liang, Lv, Yi-zhi, Ye, Jian-zhi, Zeng, Shao-dong, Wang, Xiao-fang, Deng, Xiao-hong, Ma, Hui-fang, Pan, Xiaowei, Lin, Li-yun
Format: Article
Language:English
Subjects:
Acetonitrile
Acidification
Aminoglycoside antibiotics
Aminoglycosides
Antibiotics
Carbadox
Carboxylic acids
Chromatography
Correlation coefficient
Correlation coefficients
High performance liquid chromatography
Ionization
Ions
Liquid chromatography
Litopenaeus vannamei
Mass spectrometry
Mass spectroscopy
Metabolites
Quinoxaline
Quinoxalines
Residues
Tilmicosin
Tylosin
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Summary:An ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-methylquinoxaline-2-carboxylic acid) and aminoglycoside antibiotics(Tilmicosin, Tylosin) residues in Penaeus. vannamei, Samples were extracted with acidified acetonitrile, then cleaned up with hexane. The separation of targets was carried out on a Waters BEH C18 column using acetonitrile and 0. 1% fomicacid as mobile phase. Analyte identification and quantification were performed by MS/MS under multiple-reaction monitering(MRM) mode with the external standard method. The results showed that there were good linear relationships between peak area and concentrations of nine targets in the range of 2.5 ~ 50 μg/L, with correlation coefficients more than 0. 999 and The limits of detection(LOD) were in the range of 0. 1 ~ 0.9 μg/kg. the recoveries ranged from 80.2% ~ 94.0%, with RSDs of 1.7%~8.4%. The method is simple, accurate and precise, and It could satisfy the requirement of mass and fast analysis in the laborator.
ISSN:2267-1242
2555-0403
2267-1242
DOI:10.1051/e3sconf/202021301033