Exploring the Role of a Novel Interleukin-17 Homolog from Invertebrate Marine Mussel Mytilus coruscus in Innate Immune Response: Is Negative Regulation by Mc -Novel_miR_145 the Key?

• Published in: International journal of molecular sciences 2023-03, Vol.24 (6), p.5928
• Main Authors: Chen, Xinglu, Qiu, Longmei, Si, Xirui, Zhang, Xiaolin, Guo, Baoying, Liao, Zhi, Yan, Xiaojun, Qi, Pengzhi
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Apoptosis - genetics; Assaying; Bacterial infections; Bioinformatics; Cytokines; Flow cytometry; Genes; Genomes; Gram-positive bacteria; HEK293 Cells; Homology; Humans; Immune response; Immune system; Immunity, Innate - genetics; Immunomodulation; Infections; Innate immunity; Interleukin 17; Interleukin-17 - genetics; Invertebrates; Lipopolysaccharides; Lipopolysaccharides - pharmacology; microRNA; MicroRNAs; MicroRNAs - genetics; Mytilus; Mytilus coruscus; NF-kappa B; NF-κB protein; Pathogens; Phylogenetics; Western blotting
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24065928

Interleukin-17 (IL-17) represents a class of proinflammatory cytokines involved in chronic inflammatory and degenerative disorders. Prior to this study, it was predicted that an IL-17 homolog could be targeted by -novel_miR_145 to participate in the immune response of . This study employed a variety of molecular and cell biology research methods to explore the association between -novel_miR_145 and IL-17 homolog and their immunomodulatory effects. The bioinformatics prediction confirmed the affiliation of the IL-17 homolog with the mussel IL-17 family, followed by quantitative real-time PCR assays (qPCR) to demonstrate that IL-17-3 was highly expressed in immune-associated tissues and responded to bacterial challenges. Results from luciferase reporter assays confirmed the potential of IL-17-3 to activate downstream NF-κb and its targeting by -novel_miR_145 in HEK293 cells. The study also produced IL-17-3 antiserum and found that -novel_miR_145 negatively regulates IL-17-3 via western blotting and qPCR assays. Furthermore, flow cytometry analysis indicated that -novel_miR_145 negatively regulated IL-17-3 to alleviate LPS-induced apoptosis. Collectively, the current results showed that IL-17-3 played an important role in molluscan immune defense against bacterial attack. Furthermore, IL-17-3 was negatively regulated by -novel_miR_145 to participate in LPS-induced apoptosis. Our findings provide new insights into noncoding RNA regulation in invertebrate models.