Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish

Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity...

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Bibliographic Details
Published in:NPJ microgravity 2023-08, Vol.9 (1), p.63-63, Article 63
Main Authors: Kim, Daehan, Nguyen, Que Thanh Thanh, Lee, Seungjin, Choi, Kyung-Mi, Lee, Eun-Ju, Park, Joong Yull
Format: Article
Language:English
Subjects:
3-D printers
631/67/2195
639/166/984
639/166/985
Applied Microbiology
Biomedical and Life Sciences
Biotechnology
Caveolin
Caveolin-1
Cell culture
Classical and Continuum Physics
Immunology
Life Sciences
Microgravity
Ovarian cancer
Polydimethylsiloxane
Space Exploration and Astronautics
Space Sciences (including Extraterrestrial Physics
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Summary:Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting experiments in real space, clinostats, which continuously randomize the gravity vector by using motors, have been used to generate simulated microgravity (SMG) on Earth. Herein, by using a 3D printing method, we develop a customized small-sized clinostat (CS clinostat) that is easy to manufacture, inexpensive, and robust. Moreover, we develop and fabricate a gas-permeable polydimethylsiloxane culture dish that fits inside the CS clinostat. To validate SMG generation, ovarian cancer cells (OV- 90, TOV-21G, and Caov-3) were applied to demonstrate a significant reduction in caveolin-1 expression, a biomarker of SMG, indicating SMG generation. The proposed CS clinostat system has good accessibility for SMG research, which makes it useful as a tool for biologists, who are unfamiliar with conventional clinostat equipment, to conduct preliminary studies in the space environment.
ISSN:2373-8065
2373-8065
DOI:10.1038/s41526-023-00311-1