Effects of mineral fertilization (NPK) on combined high temperature and ozone damage in rice

• Published in: BMC plant biology 2024-10, Vol.24 (1), p.974-12, Article 974
• Main Authors: Jo, So-Hye, Kim, Ju-Hee, Moon, Ji-Hyeon, Yang, Seo-Yeong, Baek, Jae-Kyeong, Song, Yeong-Seo, Shon, Ji-Young, Chung, Nam-Jin, Lee, Hyeon-Seok
• Format: Article
• Language: English
• Subjects: Abscisic acid; Age; Air pollution; Air quality management; Antioxidants; Calibration; Cellular stress response; Climate change; Combined stress; Crop damage; Crop production; Crop resilience; Crop science; Damage tolerance; Diseases and pests; Dry weight; Environmental aspects; Environmental conditions; Environmental stress; Extreme weather; Factorial experiments; Fertilization; Fertilization of plants; Fertilizers; Gene expression; Genetic aspects; Global warming; Growth; Health aspects; High temperature; Hot Temperature; Humidity; Leaves; Methods; Mineral fertilizers; Minerals - metabolism; Nitrogen; Oryza - drug effects; Oryza - growth & development; Oryza - physiology; Ozonation; Ozone; Ozone - pharmacology; Physiological aspects; Physiological effects; Physiology; Plant layout; Plants; Plants (botany); Potassium; Potassium conductance; Production management; Productivity; Protein synthesis; Proteins; Rice; Statistical analysis; Stomata; Stomatal conductance; Stress, Physiological; Surface ozone; Temperature tolerance; VOCs; Volatile organic compounds; Weight
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05695-0

Increasing concern has recently been highlighted regarding crop damage due to extreme weather events caused by global warming and the increased production of ground-level ozone. Several studies have investigated rice growth in response to fertilization conditions under various environmental stress conditions; however, studies on growth development in response to fertilization conditions under combined high-temperature/ozone treatment conditions are scarce. In this study, we aimed investigate the growth and physiological development of rice under combined high temperature and ozone treatment conditions and to reveal the damage-mitigation effects of NPK fertilization treatments. The plants were treated with varying levels of NPK [N2 (N-P-K: 9.0-4.5-4.0 kg/a), P2 (4.5-9.0-4.0 kg/a), K2 (4.5-4.5-8.0 kg/a), and control (4.5-4.5-4.0 kg/10a).] under combined high-temperature (35 ℃) and ozone (150 pb) treatment conditions. Analysis of the growth metrics, including plant height, leaf age, dry weight, and the plant height/leaf age (PH/L) ratio were revealed that combined high-temperature/ozone treatment promoted the phenological development indicated by increasing leaf age but decreased the plant height and dry weight indicating its negative effect on quantitative growth. The effects of this combined high-temperature/ozone treatment on growth were alleviated by NPK fertilization, particularly in K2 treatment but worsened in N2 treatment. Visible damage symptoms in rice leaves induced by exposure to the combined stressors was also alleviated by the K2 treatment. At the physiological level, K2 treatment reduced the expression of OsF3H2, which is associated with antioxidant activity, suggesting that potassium improved stress tolerance. Additionally, expression of genes related to abscisic acid (ABA) metabolism showed increased OsNECD (ABA synthesis) and decreased OsCYP707A3 (ABA degradation) in the K2 treatment, promoting a stronger adaptive stress response. Stomatal conductance measurements indicated a slight increase under K2 treatment, reflecting enhanced regulation of stomatal function during stress. The study highlights the potential of potassium fertilization to mitigate combined high-temperature and ozone stress in rice, suggesting it as a strategy to improve crop resilience and optimize fertilization. The findings offer insights into fertilization treatments and can guide future research on stress tolerance in crops.