Glial cell type-specific gene expression in the mouse cerebrum using the piggyBac system and in utero electroporation

Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse...

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Published in:Scientific reports 2021-03, Vol.11 (1), p.4864-4864, Article 4864
Main Authors: Hamabe-Horiike, Toshihide, Kawasaki, Kanji, Sakashita, Masataka, Ishizu, Chihiro, Yoshizaki, Tomokazu, Harada, Shin-ichi, Ogawa-Ochiai, Keiko, Shinmyo, Yohei, Kawasaki, Hiroshi
Format: Article
Language:English
Subjects:
631/378/2571
631/378/368/2430
Astrocytes
Central nervous system
Cerebrum
Electroporation
Gene expression
Glial cells
Humanities and Social Sciences
Molecular modelling
multidisciplinary
Myelin proteolipid protein
Oligodendrocytes
Science
Science (multidisciplinary)
Transgenic mice
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Summary:Glial cells such as astrocytes and oligodendrocytes play crucial roles in the central nervous system. To investigate the molecular mechanisms underlying the development and the biological functions of glial cells, simple and rapid techniques for glial cell-specific genetic manipulation in the mouse cerebrum would be valuable. Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation. In contrast, the Blbp promoter, which has been used to induce astrocyte-specific gene expression in transgenic mice, did not result in astrocyte-specific gene expression. We also identified the Plp1 and Mbp promoters could be used with the piggyBac system and in utero electroporation to induce selective gene expression in oligodendrocytes. Furthermore, using our technique, neuron-astrocyte or neuron-oligodendrocyte interactions can be visualized by labeling neurons, astrocytes and oligodendrocytes differentially. Our study provides a fundamental basis for specific transgene expression in astrocytes and/or oligodendrocytes in the mouse cerebrum.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-84210-z