Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2

The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggest...

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Bibliographic Details
Published in:Pathogens (Basel) 2023-01, Vol.12 (2), p.191
Main Authors: Ávila-Ramírez, María Leticia, Reyes-Reyes, Ana Laura, Avila-Bonilla, Rodolfo Gamaliel, Salas-Benito, Mariana, Cerecedo, Doris, Ramírez-Moreno, María Esther, Villagrán-Herrera, María Elena, Mercado-Curiel, Ricardo Francisco, Salas-Benito, Juan Santiago
Format: Article
Language:English
Subjects:
Aedes albopictus
Amplified fragment length polymorphism
Aquatic insects
Carrier proteins
Dengue
Dengue fever
Dengue virus
Dengue viruses
Development and progression
DNA polymerase
Gene expression
Genetic aspects
Genomes
Hypotheses
importin
Infections
L cells
Mosquitoes
nuclear transport
Persistent infection
Physiological aspects
Proteins
Vector-borne diseases
Viral infections
viral persistence
Viral proteins
Virus research
Viruses
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Summary:The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens12020191