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Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2
The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggest...
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| Published in: | Pathogens (Basel) 2023-01, Vol.12 (2), p.191 |
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| creator | Ávila-Ramírez, María Leticia Reyes-Reyes, Ana Laura Avila-Bonilla, Rodolfo Gamaliel Salas-Benito, Mariana Cerecedo, Doris Ramírez-Moreno, María Esther Villagrán-Herrera, María Elena Mercado-Curiel, Ricardo Francisco Salas-Benito, Juan Santiago |
| description | The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in
cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate. |
| doi_str_mv | 10.3390/pathogens12020191 |
| format | article |
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cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate.</description><identifier>ISSN: 2076-0817</identifier><identifier>EISSN: 2076-0817</identifier><identifier>DOI: 10.3390/pathogens12020191</identifier><identifier>PMID: 36839463</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Aedes albopictus ; Amplified fragment length polymorphism ; Aquatic insects ; Carrier proteins ; Dengue ; Dengue fever ; Dengue virus ; Dengue viruses ; Development and progression ; DNA polymerase ; Gene expression ; Genetic aspects ; Genomes ; Hypotheses ; importin ; Infections ; L cells ; Mosquitoes ; nuclear transport ; Persistent infection ; Physiological aspects ; Proteins ; Vector-borne diseases ; Viral infections ; viral persistence ; Viral proteins ; Virus research ; Viruses</subject><ispartof>Pathogens (Basel), 2023-01, Vol.12 (2), p.191</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c512t-78cdb6b65c54f9734c7f09afc363f6e804285054d3f1f8933082d15d8235bc5c3</cites><orcidid>0000-0002-0015-8081 ; 0000-0002-0582-0046 ; 0000-0001-7002-0881 ; 0000-0002-4096-0079</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2779626504/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2779626504?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36839463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ávila-Ramírez, María Leticia</creatorcontrib><creatorcontrib>Reyes-Reyes, Ana Laura</creatorcontrib><creatorcontrib>Avila-Bonilla, Rodolfo Gamaliel</creatorcontrib><creatorcontrib>Salas-Benito, Mariana</creatorcontrib><creatorcontrib>Cerecedo, Doris</creatorcontrib><creatorcontrib>Ramírez-Moreno, María Esther</creatorcontrib><creatorcontrib>Villagrán-Herrera, María Elena</creatorcontrib><creatorcontrib>Mercado-Curiel, Ricardo Francisco</creatorcontrib><creatorcontrib>Salas-Benito, Juan Santiago</creatorcontrib><title>Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2</title><title>Pathogens (Basel)</title><addtitle>Pathogens</addtitle><description>The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in
cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate.</description><subject>Aedes albopictus</subject><subject>Amplified fragment length polymorphism</subject><subject>Aquatic insects</subject><subject>Carrier proteins</subject><subject>Dengue</subject><subject>Dengue fever</subject><subject>Dengue virus</subject><subject>Dengue viruses</subject><subject>Development and progression</subject><subject>DNA polymerase</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Hypotheses</subject><subject>importin</subject><subject>Infections</subject><subject>L cells</subject><subject>Mosquitoes</subject><subject>nuclear transport</subject><subject>Persistent infection</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Vector-borne diseases</subject><subject>Viral infections</subject><subject>viral persistence</subject><subject>Viral proteins</subject><subject>Virus research</subject><subject>Viruses</subject><issn>2076-0817</issn><issn>2076-0817</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptUs1uEzEQXiEQrUofgAuyxIVLWq-99q4vSFFaSqQKKvFztbz2OHG0sYPtLfTIK_EgPBNOU0oD2Ad7Zr7vG894qup5jU8oFfh0o_IyLMCnmmCCa1E_qg4JbvkEd3X7-MH9oDpOaYXL6vDWflodUN5R0XB6WH0_c9ZCBJ-dGtAFeEDn3zYRUnLBoyuVM0SPgkXz9SbE7Dz6-YMi5Q1694GhYs74KeVoBsOQ0FSPGYab2_AVxORSLsLFMfcWdAaDvrq8RGfgFyOgzy6OCZFn1ROrhgTHd-dR9enN-cfZ28nl-4v5bHo50awmedJ22vS850yzxoqWNrq1WCirKaeWQ4cb0jHMGkNtbTtBKe6IqZnpCGW9ZpoeVfOdrglqJTfRrVW8kUE5eesIcSFVKVAPIHvDFCWAtYWSR5Gek9oIAqzTxPQCitbrndZm7NdgdKkyqmFPdD_i3VIuwrUUgvPy9iLw6k4ghi8jpCzXLunSROUhjEmStvxVgbZtgb78C7oKY_SlVQXVCk44w80f1EKVApy3oeTVW1E5bRtKCeeiK6iT_6DKNrB2Oniwrvj3CPWOoGNIKYK9r7HGcjuG8p8xLJwXD5tzz_g9dPQXvZrZgw</recordid><startdate>20230127</startdate><enddate>20230127</enddate><creator>Ávila-Ramírez, María Leticia</creator><creator>Reyes-Reyes, Ana Laura</creator><creator>Avila-Bonilla, Rodolfo Gamaliel</creator><creator>Salas-Benito, Mariana</creator><creator>Cerecedo, Doris</creator><creator>Ramírez-Moreno, María Esther</creator><creator>Villagrán-Herrera, María Elena</creator><creator>Mercado-Curiel, Ricardo Francisco</creator><creator>Salas-Benito, Juan Santiago</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-0015-8081</orcidid><orcidid>https://orcid.org/0000-0002-0582-0046</orcidid><orcidid>https://orcid.org/0000-0001-7002-0881</orcidid><orcidid>https://orcid.org/0000-0002-4096-0079</orcidid></search><sort><creationdate>20230127</creationdate><title>Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2</title><author>Ávila-Ramírez, María Leticia ; Reyes-Reyes, Ana Laura ; Avila-Bonilla, Rodolfo Gamaliel ; Salas-Benito, Mariana ; Cerecedo, Doris ; Ramírez-Moreno, María Esther ; Villagrán-Herrera, María Elena ; Mercado-Curiel, Ricardo Francisco ; Salas-Benito, Juan Santiago</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-78cdb6b65c54f9734c7f09afc363f6e804285054d3f1f8933082d15d8235bc5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aedes albopictus</topic><topic>Amplified fragment length polymorphism</topic><topic>Aquatic insects</topic><topic>Carrier proteins</topic><topic>Dengue</topic><topic>Dengue fever</topic><topic>Dengue virus</topic><topic>Dengue viruses</topic><topic>Development and progression</topic><topic>DNA polymerase</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Hypotheses</topic><topic>importin</topic><topic>Infections</topic><topic>L cells</topic><topic>Mosquitoes</topic><topic>nuclear transport</topic><topic>Persistent infection</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Vector-borne diseases</topic><topic>Viral infections</topic><topic>viral persistence</topic><topic>Viral proteins</topic><topic>Virus research</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ávila-Ramírez, María Leticia</creatorcontrib><creatorcontrib>Reyes-Reyes, Ana Laura</creatorcontrib><creatorcontrib>Avila-Bonilla, Rodolfo Gamaliel</creatorcontrib><creatorcontrib>Salas-Benito, Mariana</creatorcontrib><creatorcontrib>Cerecedo, Doris</creatorcontrib><creatorcontrib>Ramírez-Moreno, María Esther</creatorcontrib><creatorcontrib>Villagrán-Herrera, María Elena</creatorcontrib><creatorcontrib>Mercado-Curiel, Ricardo Francisco</creatorcontrib><creatorcontrib>Salas-Benito, Juan Santiago</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ</collection><jtitle>Pathogens (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ávila-Ramírez, María Leticia</au><au>Reyes-Reyes, Ana Laura</au><au>Avila-Bonilla, Rodolfo Gamaliel</au><au>Salas-Benito, Mariana</au><au>Cerecedo, Doris</au><au>Ramírez-Moreno, María Esther</au><au>Villagrán-Herrera, María Elena</au><au>Mercado-Curiel, Ricardo Francisco</au><au>Salas-Benito, Juan Santiago</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2</atitle><jtitle>Pathogens (Basel)</jtitle><addtitle>Pathogens</addtitle><date>2023-01-27</date><risdate>2023</risdate><volume>12</volume><issue>2</issue><spage>191</spage><pages>191-</pages><issn>2076-0817</issn><eissn>2076-0817</eissn><abstract>The establishment of persistent dengue virus infection within the cells of the mosquito vector is an essential requirement for viral transmission to a new human host. The mechanisms involved in the establishment and maintenance of persistent infection are not well understood, but it has been suggested that both viral and cellular factors might play an important role. In the present work, we evaluated differential gene expression in
cells acutely (C6/36-HT) and persistently infected (C6-L) with Dengue virus 2 by cDNA-AFLP. We observed that importin β3 was upregulated in noninfected cells compared with C6-L cells. Using RT-qPCR and plaque assays, we observed that Dengue virus levels in C6-L cells essentially do not vary over time, and peak viral titers in acutely infected cells are observed at 72 and 120 h postinfection. The expression level of importin β3 was higher in acutely infected cells than in persistently infected cells; this correlates with higher levels of NS5 in the nucleus of the cell. The differential pattern of importin β3 expression between acute and persistent infection with Dengue virus 2 could be a mechanism to maintain viral infection over time, reducing the antiviral response of the cell and the viral replicative rate.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>36839463</pmid><doi>10.3390/pathogens12020191</doi><orcidid>https://orcid.org/0000-0002-0015-8081</orcidid><orcidid>https://orcid.org/0000-0002-0582-0046</orcidid><orcidid>https://orcid.org/0000-0001-7002-0881</orcidid><orcidid>https://orcid.org/0000-0002-4096-0079</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Aedes albopictus Amplified fragment length polymorphism Aquatic insects Carrier proteins Dengue Dengue fever Dengue virus Dengue viruses Development and progression DNA polymerase Gene expression Genetic aspects Genomes Hypotheses importin Infections L cells Mosquitoes nuclear transport Persistent infection Physiological aspects Proteins Vector-borne diseases Viral infections viral persistence Viral proteins Virus research Viruses |
| title | Differential Gene Expression Pattern of Importin β3 and NS5 in C6/36 Cells Acutely and Persistently Infected with Dengue Virus 2 |
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