Prevalence and Phylogenetic Analysis of Microsporidium Enterocytozoon bieneusi in Diarrheal Patients

can cause severe diarrhea in children and adults. However, in China, there are scant studies on in diarrheal children and adults, with the exception of prevalence and genotyping data in a small number of cities including Hubei, Shanghai, and Heilongjiang. In this study, 196 fecal samples ( = 132 in...

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Bibliographic Details
Published in:Pathogens (Basel) 2021-01, Vol.10 (2), p.128
Main Authors: Zang, Manman, Li, Jinjin, Tang, Chun, Ding, Songtao, Huang, Wei, Qin, Qizhong, Liu, Handeng
Format: Article
Language:English
Subjects:
Adults
Animals
Children
Diarrhea
Drinking water
Enterocytozoon bieneusi
Epidemiology
genotype
Genotype & phenotype
Genotypes
Genotyping
HIV
Human immunodeficiency virus
Infections
microscopic examination
patient
Phylogenetics
Phylogeny
Reptiles & amphibians
Sequence analysis
Transcription
Zoonoses
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Summary:can cause severe diarrhea in children and adults. However, in China, there are scant studies on in diarrheal children and adults, with the exception of prevalence and genotyping data in a small number of cities including Hubei, Shanghai, and Heilongjiang. In this study, 196 fecal samples ( = 132 in Chongqing, = 44 in Shandong, = 20 in Hubei) were collected, including 91 from children and 105 from adults. Through microscopic examination, 19 positive samples (11 from children and 8 from adults) were detected. Using PCR examination, the internal transcriptional spacer (ITS) region was utilized by nested PCR to detect and characterize . Twenty positive samples were detected, including 14 from children (≤11 years of age) and 6 from adults. According to the sequence analysis of ITS data, one known zoonotic (D) and seven novel (CQH5-11) genotypes were identified. This is the first molecular epidemiological study of in diarrheal patients in different regions of China. Therefore, this study can provide useful information for the molecular epidemiology and control of infection in humans in the future.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens10020128