Transcription Factor IAA27 Positively Regulates P Uptake through Promoted Adventitious Root Development in Apple Plants

• Published in: International journal of molecular sciences 2022-11, Vol.23 (22), p.14029
• Main Authors: Zhao, Shuo, Zhao, Xuewen, Xu, Xuefeng, Han, Zhenhai, Qiu, Changpeng
• Format: Article
• Language: English
• Subjects: Abiotic stress; Acetic acid; adventitious root; Agricultural production; Auxins; Gene expression; Gene Expression Regulation, Plant; Indoleacetic acid; Indoleacetic Acids - metabolism; Investigations; low phosphorus stress; Malus - genetics; Malus - metabolism; Malus domestica; MdIAA27; Molecular modelling; Mutation; Phosphorus; Phosphorus - metabolism; Phylogenetics; Plant growth; Plant Roots - metabolism; Proteins; Tobacco; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232214029

Phosphate (P) deficiency severely limits the growth and production of plants. Adventitious root development plays an essential role in responding to low phosphorus stress for apple plants. However, the molecular mechanisms regulating adventitious root growth and development in response to low phosphorus stress have remained elusive. In this study, a mutation (C-T) in the coding region of the apple gene was identified. -overexpressing transgenic apple improved the tolerance to phosphorus deficiency, which grew longer and denser adventitious roots and presented higher phosphorous content than the control plants under low phosphorus conditions, while the overexpression of displayed the opposite trend. Moreover, the heterologous overexpression of in tobacco yielded the same results, supporting the aforementioned findings. In vitro and in vivo assays showed that MdIAA27 directly interacted with AUXIN RESPONSE FACTOR (ARF8), ARF26 and ARF27, which regulated and transcription. The mutation in IAA27 resulted in altered interaction modes, which in turn promoted the release of positive ARFs to upregulate and expression in low phosphorus conditions. Altogether, our studies provide insights into how the allelic variation of affects adventitious root development in response to low phosphorus stress.