Chemical Variability and In Vitro Anti-Inflammatory Activity of Leaf Essential Oil from Ivorian Isolona dewevrei (De Wild. & T. Durand) Engl. & Diels

• Published in: Molecules (Basel, Switzerland) Switzerland), 2021-10, Vol.26 (20), p.6228
• Main Authors: Kambiré, Didjour Albert, Boti, Jean Brice, Kablan, Ahmont Claude Landry, Ballo, Daouda, Paoli, Mathieu, Brunini, Virginie, Tomi, Félix
• Format: Article
• Language: English
• Subjects: Annonaceae - chemistry; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; Caryophyllene; chemical variability; Chromatography; Constituents; Cote d'Ivoire; Drug Evaluation, Preclinical; Essential oils; Eudesmol; Gas Chromatography-Mass Spectrometry; Germacrene; Glycine max - enzymology; in vitro anti-inflammatory activity; In Vitro Techniques; Inflammation; Isolona dewevrei; leaf essential oil; Leaves; Lipoxygenase; Lipoxygenase - drug effects; Lipoxygenase Inhibitors - chemistry; Lipoxygenase Inhibitors - isolation & purification; Lipoxygenase Inhibitors - pharmacology; Magnetic Resonance Spectroscopy; NMR; Nuclear magnetic resonance; Ocimene; Oils & fats; Oils, Volatile - chemistry; Oils, Volatile - classification; Oils, Volatile - pharmacology; Plant Leaves - chemistry; Plant Oils - chemistry; Plant Oils - classification; Plant Oils - pharmacology; Plants, Medicinal - chemistry; Subgroups
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26206228

The chemical variability and the in vitro anti-inflammatory activity of the leaf essential oil from Ivorian were investigated for the first time. Forty-seven oil samples were analyzed using a combination of CC, GC(RI), GC-MS and C-NMR, thus leading to the identification of 113 constituents (90.8-98.9%). As the main components varied drastically from sample to sample, the 47 oil compositions were submitted to hierarchical cluster and principal components analyses. Three distinct groups, each divided into two subgroups, were evidenced. Subgroup I-A was dominated by ( )-β-ocimene, β-eudesmol, germacrene D and ( )-β-ocimene, while (10βH)-1β,8β-oxido-cadina-4-ene, santalenone, -α-bergamotene and -β-bergamotene were the main compounds of Subgroup I-B. The prevalent constituents of Subgroup II-A were germacrene B, ( )-β-caryophyllene, (5αH,10βMe)-6,12-oxido-elema-1,3,6,11(12)-tetraene and γ-elemene. Subgroup II-B displayed germacrene B, germacrene D and ( )-β-ocimene as the majority compounds. Germacrene D was the most abundant constituent of Group III, followed in Subgroup III-A by ( )-β-caryophyllene, (10βH)-1β,8β-oxido-cadina-4-ene, germacrene D-8-one, and then in Subgroup III-B by ( )-β-ocimene and ( )-β-ocimene. The observed qualitative and quantitative chemical variability was probably due to combined factors, mostly phenology and season, then harvest site to a lesser extent. The lipoxygenase inhibition by a leaf oil sample was also evaluated. The oil IC (0.020 ± 0.005 mg/mL) was slightly higher than the non-competitive lipoxygenase inhibitor NDGA IC (0.013 ± 0.003 mg/mL), suggesting a significant in vitro anti-inflammatory potential.