Role of Polyphenols from the Aqueous Extract of Aloysia citrodora in the Inhibition of Aflatoxin B1 Synthesis in Aspergillus flavus

Aflatoxin B1 (AFB1) is a mycotoxin considered a potent carcinogen for humans that contaminates a wide range of crops. Various strategies have been established to reduce or block the synthesis of AFB1 in food and feed. The use of aqueous extracts derived from plants with high antioxidant activity has...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2023-06, Vol.28 (13), p.5123
Main Authors: Cadenillas, Laura F, Hernandez, Christopher, Bailly, Sylviane, Billerach, Guillaume, Durrieu, Vanessa, Bailly, Jean-Denis
Format: Article
Language:English
Subjects:
Aflatoxin B1
Aflatoxins
Aloysia citrodora
Antioxidants
aqueous extract
Aspergillus flavus
Carcinogens
Flavonoids
Fractionation
High performance liquid chromatography
Humans
Hypotheses
Life Sciences
Liquid chromatography
luteolin-7-diglucuronide
Mass spectrometry
Mass spectroscopy
Morphology
Mycotoxins
Oxidative stress
Plant extracts
Polyphenols
Polyphenols - pharmacology
Toxins
Verbenaceae
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Summary:Aflatoxin B1 (AFB1) is a mycotoxin considered a potent carcinogen for humans that contaminates a wide range of crops. Various strategies have been established to reduce or block the synthesis of AFB1 in food and feed. The use of aqueous extracts derived from plants with high antioxidant activity has been a subject of study in recent years due to their efficacy in inhibiting AFB1. In this study, we assessed the effect of aqueous extract on growth and on AFB1 production. A bio-guided fractionation followed by High Performance Liquid Chromatography (HPLC) and Mass spectrometry analysis of the active fraction were applied to identify the candidate molecules responsible for the dose-effect inhibition of AFB1 synthesis. Our results revealed that polyphenols are the molecules implicated in AFB1 inhibition, achieving almost a total inhibition of the toxin production (99%). We identified luteolin-7-diglucuronide as one of the main constituents in extract, and demonstrated that it is able to inhibit, by itself, AFB1 production by 57%. This is the first study demonstrating the anti-Aflatoxin B1 effect of this molecule, while other polyphenols surely intervene in anti-AFB1 activity.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28135123