Microcystin-Detoxifying Recombinant Saccharomyces cerevisiae Expressing the mlrA Gene from Sphingosinicella microcystinivorans B9

Contamination of water by microcystins is a global problem. These potent hepatotoxins demand constant monitoring and control methods in potable water. Promising approaches to reduce contamination risks have focused on natural microcystin biodegradation led by enzymes encoded by the genes. The first...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-02, Vol.11 (3), p.575
Main Authors: Silva, Fernando de Godoi, Lopes, Daiane Dias, Hector, Ronald E, Nascimento, Maikon Thiago do, Miguel, Tatiana de Ávila, Kuroda, Emília Kiyomi, Nóbrega, Gisele Maria de Andrade de, Harada, Ken-Ichi, Hirooka, Elisa Yoko
Format: Article
Language:English
Subjects:
Biodegradation
Cloning
Contamination
Control methods
Cultivation
Drinking water
E coli
Enzymes
Gene expression
Genetic engineering
Hepatotoxicity
heterologous expression
microcystinase
Microcystins
Microorganisms
Plasmids
public health
Saccharomyces cerevisiae
Toxicity
Toxins
Water pollution
Water treatment
Yeast
Yeasts
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Summary:Contamination of water by microcystins is a global problem. These potent hepatotoxins demand constant monitoring and control methods in potable water. Promising approaches to reduce contamination risks have focused on natural microcystin biodegradation led by enzymes encoded by the genes. The first enzyme of this system ( ) linearizes microcystin structure, reducing toxicity and stability. Heterologous expression of in different microorganisms may enhance its production and activity, promote additional knowledge on the enzyme, and support feasible applications. In this context, we intended to express the gene from B9 in an industrial strain as an innovative biological alternative to degrade microcystins. The gene was codon-optimized for expression in yeast, and either expressed from a plasmid or through chromosomal integration at the locus. Recombinant and wild yeasts were cultivated in medium contaminated with microcystins, and the toxin content was analyzed during growth. Whereas no difference in microcystins content was observed in cultivation with the chromosomally integrated strain, the yeast strain hosting the expression plasmid reduced 83% of toxins within 120 h of cultivation. Our results show microcystinase A expressed by industrial yeast strains as a viable option for practical applications in water treatment.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11030575