Cyanide Insensitive Oxidase Confers Hydrogen Sulfide and Nitric Oxide Tolerance to Pseudomonas aeruginosa Aerobic Respiration

• Published in: Antioxidants 2024-03, Vol.13 (3), p.383
• Main Authors: Nastasi, Martina R, Caruso, Lorenzo, Giordano, Francesca, Mellini, Marta, Rampioni, Giordano, Giuffrè, Alessandro, Forte, Elena
• Format: Article
• Language: English
• Subjects: Aerobic respiration; Antibiotics; Bacteria; bd-type terminal oxidases; Copper; cyanide insensitive oxidase; Cyanides; Cystic fibrosis; Cytochrome; E coli; Electron transport; Enzymes; Genes; Hydrogen sulfide; Immune response; Immunological tolerance; Infections; Metabolism; Nitric oxide; Opportunist infection; Oxidative stress; Oxygen consumption; Pathogens; Physiology; Proteins; Pseudomonas aeruginosa; Respiration; respiratory chain; Virulence
• ISSN: 2076-3921; 2076-3921
• DOI: 10.3390/antiox13030383

Hydrogen sulfide (H S) and nitric oxide (NO) are long-known inhibitors of terminal oxidases in the respiratory chain. Yet, they exert pivotal signaling roles in physiological processes, and in several bacterial pathogens have been reported to confer resistance against oxidative stress, host immune responses, and antibiotics. , an opportunistic pathogen causing life-threatening infections that are difficult to eradicate, has a highly branched respiratory chain including four terminal oxidases of the haem-copper type ( , , , and ) and one oxidase of the -type (cyanide-insensitive oxidase, CIO). As -type oxidases have been shown to be H S-insensitive and to readily recover their activity from NO inhibition, here we tested the effect of H S and NO on CIO by performing oxygraphic measurements on membrane preparations from PAO1 and isogenic mutants depleted of CIO only or all other terminal oxidases except CIO. We show that O consumption by CIO is unaltered even in the presence of high levels of H S, and that CIO expression is enhanced and supports bacterial growth under such stressful conditions. In addition, we report that CIO is reversibly inhibited by NO, while activity recovery after NO exhaustion is full and fast, suggesting a protective role of CIO under NO stress conditions. As is exposed to H S and NO during infection, the tolerance of CIO towards these stressors agrees with the proposed role of CIO in virulence.