Point-of-Care Testing – Biosensor for Norepinephrine Determination

An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of l...

Full description

Saved in:
Bibliographic Details
Published in:International Journal of Electronics and Telecommunications 2020-01, Vol.66 (2), p.369-372
Main Authors: Baluta, Sylwia, Swist, Agnieszka, Cabaj, Joanna, Malecha, Karol
Format: Article
Language:English
Subjects:
biosensor
Biosensors
Carbazoles
Catalytic oxidation
Catecholamine
Coated electrodes
cyclic voltammetry
Laccase
Norepinephrine
Oxidation
point-of-care
Polymer films
Quinones
Selectivity
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An useful electrochemical sensing approach was developed for norepinephrine (NE) detection based on semiconducting polymer (9-nonyl-2,7-di(selenophen-2-yl)- 9H-carbazole) and laccase modified platinum electrode (Pt). The miniature Pt biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with thin polymeric film. This sensing arrangement utilized the catalytic oxidation of NE to norepinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of enzyme – laccase. With the optimized conditions, the analytical performance demonstrated selectivity in a wide linear range (0.1–200x10-6 M) with a detection limit of 240 nM and a quantification limit of 365 nM. Moreover, the method was successfully applied for selective NE determination in the presence of interfering substances.
ISSN:2081-8491
2300-1933
DOI:10.24425/ijet.2020.131887